Using a laser trap, we have studied the properties of erythrocytes from a sickle cell anemia patient (SCA) after receiving an intravenous blood transfusion, and a normal adult individual carrying normal adult hemoglobin. The hemoglobin type and quantitation assessment was carried out by high performance liquid chromatography (HPLC). We conducted an analysis of the size distributions of the cell...

By KARL SINGER, BASIL ANGELOPOULOS AND BRACHA RAMOT I T IS N(.)W well established that the erythrocytes of normal newborns contain about 50 to 90 per cent of fetal (type F) hemoglobin, the remainder being supplied by the normal adult (type A) pigment. As a rule, the fetal hemoglobin is rapidly replaced by the adult compound and after 7 to 12 months the embryonic pigment is no longer demonstrabl...

BACKGROUND Vitamin B12 deficiency is highly prevalent among adult individuals with diabetes yet screening is infrequent in Uganda. There are currently no published data regarding the prevalence of vitamin B12 deficiency and its associated factors among adult individuals with diabetes in sub-Saharan Africa. This study aimed at describing the prevalence and factors associated with vitamin B12 def...

H UMAN and several other mammalian species sequentially express different globin genes during ontogeny, or during periods of “stress,” such as hypoxemia or anemia. Hemoglobin ontogeny in humans includes three distinct phases. The “embryonic” phase predominates from the 5th to the 16th week of gestation. In this period, embryonic hemoglobin chains are and . Throughout the remainder of gestation ...

The Malarial parasite resides in the host RBC during its erythrocytic cycle. Plasmodium meets its entire nutritional requirement from RBC. It scavenges the hemoglobin of RBCs to meet its amino acid requirement. The host hemoglobin is made of different chains and it is dependent on age. Hemoglobin F (HbF), which has two-alpha and two gamma chain persists in children upto six years, and hemoglobi...

In an English family living in the county of Norfolk, England, Ager, Lehmann, and Vella (2) have described the occurrence of an electrophoretically fast moving variant of human adult hemoglobin (hemoglobin A). This hemoglobin has been named hemoglobin Norfolk. Gammack et al. (3) have reported that hemoglobin Norfolk contains abnormal ac-peptide chains. By a microscale adaptation of the dissocia...

K562 human leukemia cells synthesize embryonic hemoglobins after culture in the presence of hemin. We have rigorously identified these hemoglobins by globin chain analysis and peptide mapping. No adult hemoglobin could be detected, and beta-globin synthesis was less than 2 ppm of total protein synthesis. Persistent embryonic globin gene expression is known to occur as a consequence of globin ge...

The regulation of fetal hemoglobin in adult erythroid cells was investigated with bone marrow cultures. Fetal hemoglobin (Hb F) was identified in individual erythroid colonies with fluorescent antibodies against Hb F and synthesis of gamma chains was determined with analyses of radioactive globins. The appearance of fetal hemoglobin in erythroid colonies was clonal. All the cells of the Hb F sy...