نتایج جستجو برای: affinity purification

تعداد نتایج: 136850  

2002
P. Ramage R. Hemmig B. Mathis S. W. Cowan - Jacob J. M. Rondeau J. Kallen M. J. J. Blommers M. Zurini S. Rüdisser

1 The unusual behavior during purification of four affinity-tagged proteins—a MAP kinase, a transcription factor, intracellular binding protein, and a receptor tyrosine kinase—led the authors to devise alternate methods of obtaining their end-product. They found it necessary to either remove the affinity tag (by recloning or by cleavage of the tag) or omit the traditional immobilized metal ion ...

Journal: :European Journal of Biochemistry 1970

Journal: :Protein expression and purification 1997
J Nilsson S Ståhl J Lundeberg M Uhlén P A Nygren

Genetic engineering enables the construction of gene man Genome Project (5), efficient and robust production and purification strategies are necessary (6). For fusions resulting in fusion proteins having the combined properties of the original gene products. Fusion industrial production of recombinant proteins, simple and fast purification methods introduced as an early can be made on either or...

Journal: :Journal of the agricultural chemical society of Japan 1988

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