A total of 540 one-day old male broilers Ross 308 were allocated to 6 treatments in a completely randomized designed with a 2×3 factorial arrangement (aflatoxin B1 (AFB1) and probiotics) with 6 replicates and 15 birds in each. Factors include aflatoxin B1 (control, 500 ppb) and probiotics (control, 108 cfu/mL Bacillus sp. MBIA2.40, 0.2 g Gallipro per kg). Results showed that adding 500 ppb AFB1...

A variety of moulds routinely infect the world’s cereal crops. Under certain field or storage conditions, some moulds can produce toxin metabolites “mycotoxins“. Aflatoxins (AFs) are a group of highly carcinogenic mycotoxins produced primarily by the fungus Aspergillus flavus. Within the group of AFs, Aflatoxin B1 (AFB1) have been reported to be carcinogenic, teratogenic, tremorgenic, and derma...

Almonds are among the commodities at risk of aflatoxin contamination by Aspergillus flavus. Temperature and water activity are the two key determinants in pre and post-harvest environments influencing both the rate of fungal spoilage and aflatoxin production. Varying the combination of these parameters can completely inhibit or fully activate the biosynthesis of aflatoxin, so it is fundamental ...

A sensitive electrochemical molecularly-imprinted sensor was developed for the detection of aflatoxin B1 (AFB1), by electropolymerization of p-aminothiophenol-functionalized gold nanoparticles in the presence of AFB1 as a template molecule. The extraction of the template leads to the formation of cavities that are able to specifically recognize and bind AFB1 through π-π interactions between AFB...

Aflatoxins and melanins are the products of a polyketide biosynthesis. In this study, the search of potential inhibitors of the aflatoxin B1 (AFB1) biosynthesis was performed among compounds blocking the pigmentation in fungi. Four compounds-three natural (thymol, 3-hydroxybenzaldehyde, compactin) and one synthetic (fluconazole)-were examined for their ability to block the pigmentation and AFB1...

Organically produced spices and herbs were analyzed for determination of aflatoxin B1 (AFB1) by ELISA using immunoaffinity column. For this purpose 93 organic spices and 37 organic herbs were randomly selected from organic markets and organic shops in Turkey. AFB1 was detected in 58 organic spice and 32 organic herb samples. Among organic spice samples, the maximum value was detected in cinnamo...

Although the aflatoxins were discovered more than 30 years ago, there is still considerable controversy surrounding their human health effects. Most countries have introduced legislation to control the level of aflatoxins in food, but it is not known if these permitted levels still pose a significant cancer risk. Furthermore, it is unlikely that all the sources of human aflatoxin exposure have ...

introduction: aflatoxin b1 (afb1) is one of the most well-known hepatocarcinogens in humans. contamination of raw materials, used in the production of sausages and burgers, with aflatoxin producing molds can lead to increased level of aflatoxin in the final products and can impose hazards to human health. unfortunately, aflatoxin is resistant to heating and freezing processes, etc. and can rema...

Antibody based techniques are widely used for the detection of aflatoxins which are potent toxins with a high rate of occurrence in many crops. We developed a murine monoclonal antibody of immunoglobulin A (IgA) isotype with a strong binding affinity to aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), aflatoxin G2 (AFG2) and aflatoxin M1 (AFM1). The antibody was effectively used i...