A simple, fast, reliable, and inexpensive chemical cleanup procedure was developed for quantitation of aflatoxins in major important agricultural commodities by liquid chromatography (LC). Aflatoxins were extracted from a ground sample with methanol-water (80 + 20, v/v), and after a single cleanup step on a minicolumn packed with basic aluminum oxide, they were quantitated by LC equipped with a...

The conversion of O-methylsterigmatocystin (OMST) and dihydro-O-methylsterigmatocystin to aflatoxins B1, G1, B2, and G2 requires a cytochrome P-450 type of oxidoreductase activity. ordA, a gene adjacent to the omtA gene, was identified in the aflatoxin-biosynthetic pathway gene cluster by chromosomal walking in Aspergillus parasiticus. The ordA gene was a homolog of the Aspergillus flavus ord1 ...

Aflatoxins are toxic metabolites of some Aspergillus flavus, A. parasiticus and A. nomius strains that occur in many foods and feeds. There are four major natural occurring aflatoxins: B1, B2, G1 and G2. These toxins can cause illness in human beings and animals. Aflatoxin B1 is the most abundant and toxic member of the family, and it is also the most potent hepatocarcinogen known. In order to ...

background there are different types of aflatoxins, including aflatoxin m1, m2, g1, g2, b1 and b2 produced in the food chain, especially when the food is infected with fungi and can directly be consumed by humans. considering that our country is located in the subtropical geographic region where temperature and humidity ecological factors are among determining factors in contamination with afm1...

The presence of aflatoxin in food and feed represents a significant health hazard to both human animal populations, hence exerting adverse effects on the environment global economy. maize variety, agroecological zones, control measures contamination were tested using 2x2x3 factorial randomised complete block design (RCBD). treatments consisted two varieties maize, three measures. aflasafe was a...

Aim: The aim of the study was to evaluate presence Heavy metal, Aflatoxin, Pesiticide residue and Microbial contamination Siddha herbal formulation Muppirandai Chooranam (MRC).
 Place study: metal analysis, Aflatoxin assay, residue, analysis were conducted at Noble Research Solutions, Kolathur, Chennai -99.
 Methodology: prepared as per Good Manufacturing Practices(GMP) guidelines -99...