The sugarcane borer moth, Diatraea saccharalis, is widespread throughout the Western Hemisphere, and is considered an introduced species in the southern United States. Although this moth has a wide distribution and is a pest of many crop plants including sugarcane, corn, sorghum and rice, it is considered one species. The objective was to investigate whether more than one introduction of D. sac...

Cytoplasmic male sterility (cms) in rye (Secale cereale L.), especially cytoplasma PAMPA, is used commercially in hybrid breeding programmes. The development of molecular markers that are tightly linked to the numerous genes coding for pollen fertility is expected to have great impact in the field. Morphological and cytological analyses of plants from a three-way cross C394: [(S67P/94 x S38/94)...

Turbot is one of the most important farmed fish in Europe. This species exhibits a considerable sexual dimorphism in growth and sexual maturity that makes the all-female production recommended for turbot farming. Our knowledge about the genetic basis of sex determination and the molecular regulation of gonad differentiation in this species is still limited. Our goal was to identify and compare ...

Abstract Quambalaria are fungal pathogens of Corymbia , Eucalyptus and related genera Myrtaceae. They smut fungi (Ustilaginomycota) described from structures that resemble conidia conidiophores. Whether these spore forms have asexual or sexual roles in life cycles is unknown. An epidemic Q. pitereka destroyed plantations New South Wales Queensland (Australia) 2008. We sampled 177 individuals th...

Over the last two decades advances in molecular genetics have introduced a new generation of molecular markers for the genetic improvement of livestock. This study is focused on the most advanced DNA-based molecular markers which have acted as versatile tools and have found their own position in various fields. Ever since their development, they are constantly being modified to enhance their ut...

We report the development of a Diversity Arrays Technology (DArT) marker panel and its utilisation in the development of an integrated genetic linkage map of white lupin (Lupinus albus L.) using an F8 recombinant inbred line population derived from Kiev Mutant/P27174. One hundred and thirty-six DArT markers were merged into the first genetic linkage map composed of 220 amplified fragment length...

Red or variegated chicory (Cichorium intybus L., 2n=2x=18) native to, and very extensively cultivated in North-Eastern Italy as a leafy vegetable, locally called “radicchio”, includes different types which represent valuable high-quality crops. The five major types of radicchio cultivated in the Veneto region were investigated by PCR-derived molecular markers (e.g. RAPDs, AP-PCRs, I-SSRs and AF...

Amplified fragment length polymorphisms (AFLPs) were used to characterize the genotypic diversity of a total of 114 Gallibacterium anatis isolates originating from a reference collection representing 15 biovars from four countries and isolates obtained from tracheal and cloacal swab samples of chickens from an organic, egg-producing flock and a layer parent flock. A subset of strains was also c...

PREMISE OF THE STUDY Assessing genetic distinctness or redundancy is an important part of plant germplasm characterization. We previously introduced a new marker-based approach using the average dissimilarity of an accession to assess genetic distinctness or redundancy. However, this approach has not been widely applied, largely due to the lack of software to integrate separate analyses involvi...

Fluorescently labeled size standards are indispensable for genotyping via an automated DNA sequencer. Microsatellites, amplified fragment-length polymorphisms (AFLPs), single nucleotide polymorphisms (SNPs), and random amplified polymorphic DNA (RAPD) can all be assayed on platforms such as the ABI Prism® 377 DNA Sequencer or the ABI Prism 3100-Avant Genetic Analyzer (both from Applied Biosyste...