The aim of this study was to evaluate the performance of the RIDA® Quick Cryptosporidium/Giardia-Combi test (R-Biopharm AG) as a rapid method for the routine diagnosis of giardiasis in dogs. Compared to an ELISA coproantigen test (Prospect® Giardia Microplate Assay)), the immunochromatographic test had a sensitivity of 83 %, a specificity of 79 %, a positive predictive value of 33 %, and a nega...

BACKGROUND & AIMS An increasing number of physicians use repeated measurements of stool calprotectin to monitor intestinal inflammation in patients with inflammatory bowel diseases (IBDs). A lateral flow-based rapid test allows patients to measure their own stool calprotectin values at home. The test comes with a software application (IBDoc; Bühlmann Laboratories AG, Schönenbuch, Switzerland) t...

The aim of the present study was to determine the prevalence of Dirofilaria immitis in stray, pet, and working dogs (n = 413, 266, and 103, resp.) from Guwahati (Assam) and Aizawl (Mizoram), areas located in two Northeastern States of India. Diagnostic methods applied were microscopy (wet film and Knott's concentration technique), immunological test (Ag ELISA by SNAP 4Dx ELISA kit), and molecul...

Abstract Globally, the COVID-19 pandemic has had extreme consequences for healthcare system and led to calls diagnostic tools monitor understand transmission, pathogenesis, epidemiology, as well evaluate future vaccination strategies. In this study, we have developed novel, our knowledge, flexible ELISA-based assays specific detection of human SARS-CoV-2 Abs against receptor-binding domain, inc...

To date, the value of fasting plasma acylated ghrelin (AG) and unacylated ghrelin (UAG) as potential novel biomarkers in patients with neuroendocrine tumors (NETs) is unknown. The aims of this study are to (i) compare fasting AG and UAG levels between nonobese, nondiabetic NET patients (N=28) and age- (±3 years) and sex-matched nonobese, nondiabetic controls (N=28); and (ii) study the relations...

BACKGROUND Advanced glycation end products (AGEs) have been proposed to be involved in pulmonary fibrosis, but its role in this process has not been fully understood. To investigate the role of AGE formation in pulmonary fibrosis, we used a bleomycin (BLM)-stimulated rat model treated with aminoguanidine (AG), a crosslink inhibitor of AGE formation. METHODS Rats were intratracheally instilled...

We performed a scanning mutagenesis study of heavy chain complementarity-determining region (CDR) residues to identify how mutations affected binding of the anti-carcinoma mAb BR96 to Ag, Lewis Y, and to an anti-Id Ab (anti-Id). By ELISA, we demonstrated that the anti-Id bound close to the Ag binding site of BR96, but the anti-Id and Ag sites were not identical. Immunoblot analysis and screenin...

Introduction: Viral hepatitis is one of the causes of premature death in world peoples, according to WHO reports, five percentage of people are carrier, lethal from this disease is 1%, therefore about 1-1.5 million people each year die from complications of this disease. This disease varies from an acute to chronic illness. The aim of this study was to determine the level of anti-HBs Ag (hepati...

INTRODUCTION The objective of this study was to compare the clinical usefulness of the new anti-double-stranded DNA nucleosome-complexed enzyme-linked immunosorbent assay (Anti-dsDNA-NcX ELISA), which is based on dsDNA-loaded nucleosomes as antigens, with established test systems based on dsDNA or nucleosomes alone for systemic lupus erythematosus (SLE) diagnostics and determination of disease ...

An ante mortem antigen-ELISA-based diagnosis of Taenia saginata cysticercosis was studied in artificially (n = 24) and naturally (n = 25) infected cattle with the objective of further validating the assay as a field diagnostic test. Based on total dissection as the definitive method of validity, the assay minimally detected 14 live cysticerci in artificially infected calves and 2 in naturally i...