Most dairy farms in Wisconsin continue to follow a fairly generalized formula of how to produce milk. Cows and replacement heifers are fed primarily homegrown feed from crop rotations comprising alfalfa (Medicago sativa L.), corn (Zea mays L.), oats (Avena spp.) and soybean (Glycine max L. Merr.). Protein and mineral supplements are purchased to compliment dairy diets. However, the dairy indust...

Large amounts of flue gas desulfurization products (FGDs) are produced when SO2 emissions are trapped in the coal burning process for generation of electricity. FGDs are normally discarded instead of being reused, and reuse on soils could be important in overall management of these products. Glasshouse experiments were conducted to determine effects of various levels of three FGDs (a FGD gypsum...

The increased production and use of nanoparticles (NPs) has generated concerns about their impact on living organisms. In this study, nCu, bulk Cu, nCuO, bulk CuO, Cu(OH)2 (CuPRO 2005, Kocide 3000), and CuCl2 were exposed for 15 days to 10 days-old hydroponically grown lettuce (Lactuca sativa) and alfalfa (Medicago sativa). Each compound was applied at 0, 5, 10, and 20 mg L(-1). At harvest, we ...

Biotic interactions can improve agricultural productivity without costly and environmentally challenging inputs. Micromonospora strains have recently been reported as natural endophytes of legume nodules but their significance for plant development and productivity has not yet been established. The aim of this study was to determine the diversity and function of Micromonospora isolated from Med...

Increase in 4-coumaryl alcohol (H) units during lignification in alfalfa (Medicago sativa) alters extractability and molecular weight of lignin Angela Ziebell, Kristen Gracom, Rui Katahira, Fang Chen, Yunqiao Pu, Art Ragauskas, Richard A. Dixon and Mark Davis 1-National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401-3393; 2-Bioenergy Science Center, National Renewable ...

Alfalfa (Medicago sativa L.) is one of the most important perennial forage crops on cultivated fields in the world. Agronomical interests for this crop are based on its numerous advantageous characteristics such as (1) high yield potential and suitable feeding value, (2) different methods of utilisation green mass, hay, silage, greenchop, briquette, pellets, etc., (3) favourable environmental i...

Amylase was found in high activity (193 international units per milligram protein) in the tap root of alfalfa (Medicago sativa L. cv. Sonora). The activity was separated by gel filtration chromatography into two fractions with molecular weights of 65,700 (heavy amylase) and 41,700 (light amylase). Activity staining of electrophoretic gels indicated the presence of one isozyme in the heavy amyla...

Roundup-Ready® alfalfa was released in June of 2005, the first trait to be commercialized in alfalfa (Medicago sativa L.) using biotechnology. Traditional alfalfa varieties will likely be grown in close proximity to these new biotech varieties. While most users will probably not be sensitive to the presence of these varieties, other markets (particularly export and organic) will request hay tha...

Alfalfa (Medicago sativa L., var. El Unico) leaf discs, floating on buffer containing NH(4)Cl and photosynthesizing with (14)CO(2), produced more labeled amino acid and less sucrose than did control discs (no added NH(4)Cl). The level of pyruvate increased and that of phosphoenolpyruvate decreased. These and other changes in levels of labeled compounds led us to conclude that pyruvate kinase wa...

Diamines and polyamines are ubiquitous components of living cells, and apparently are involved in numerous cellular and physiological processes. Certain "uncommon" polyamines have limited distribution in nature and have been associated primarily with organisms adapted to extreme environments, although the precise function of these polyamines in such organisms is unknown. This article summarizes...