The X-ray synchrotron is quite different from conventional radiation sources. This technique may expand the capabilities of conventional radiology and be applied in novel manners for special cases. To evaluate the usefulness of X-ray synchrotron radiation systems for real time observations, mouse fetal skeleton development was monitored with a high resolution X-ray synchrotron. A non-monochroma...

OBJECTIVE To investigate the role and possible mechanism of α-Klotho in the calcification and the osteogenic transition of cultured VSMCs. METHODS VSMCs were cultured in vitro and divided into 5 groups, each using a different medium: (1) control; (2) β-GP; (3) β-GP + Klotho; (4) β-GP + LiCl; (5) β-GP + Klotho + LiCl. Calcium deposits were visualized using Alizarin Red S staining. The calcium ...

AIM To compare the mineralization inductive capacity of Biodentine and Bioaggregate with Mineral trioxide aggregate (MTA) and to investigate possible signaling pathways of mineralization in human dental pulp cells (HDPCs). METHODOLOGY Viability of HDPCs in response to Biodentine, Bioaggregate, and MTA was measured using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide. To investig...

Osteogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs) is accurately regulated by essential transcription factors and signaling cascades. However, the precise mechanisms involved in this process remain unknown. MicroRNAs (miRNAs) regulate various biological processes by binding target mRNA to attenuate protein synthesis. The purpose of this study was to investigate the role o...

OBJECTIVE The aim of this study was to investigate whether radiopaque Portland cement (RPC) facilitates the mineralization process in human dental pulp cells (HDPCs) compared with pure Portland cement (PC). STUDY DESIGN Under a scanning electron microscope (SEM), cellular morphology was evaluated. Alkaline phosphatase (ALP) activity was analyzed, and nodule formation was assessed by performin...

Our study aimed at investigating the effect of different molecular weight bovine collagen peptides, namely CH878, CH1370, CH2900, and CH7747 on the differentiation of MC3T3-E1 cells. Osteogenic differentiation of MC3T3-E1 cells was assessed by a series of specific assays, after culturing of cells in the presence of collagen peptides. Alkaline phosphatase activity (ALP) was evaluated by NBT/BCIP...

Highly ordered TiO2 nanotube arrays on Ti substrates were prepared by an electrochemical anodization process in a mixture of glycerol and water containing 0.5 wt% NH4F and then thermally annealed from 400 C to 600 C. The sample structures were characterized by scanning electron microscopy and X-ray diffraction measurements, where the nanotube pore diameter and length were found to increase with...

carbonates are among the most common soil constituents in arid and semiarid zones. they exhibit many significant differences in their chemical and mineralogical, suite, origin, physical and micromorphological properties and consequently in their activities in soil. the differences cause different properties of calcareous soils. six profiles of aridisols and inceptisols with carbonate accumulati...

Mesenchymal stem cells are pluripotent progenitors that could be found in human bone marrow. Mesenchymal stem cells are capable of renewing themselves without differentiation in long-term culture. These cells also have low immunogenicity and can suppress alloreactive T cell responses. In the current study, mesenchymal stem cells isolated and propagated previously from the bone marrow of a megal...