Abstract To investigate the influence of internal micellar structure on course enzymatic cross-linking especially in initial phase reaction, casein micelles isolated from raw milk via ultracentrifugation were incubated with microbial transglutaminase (mTG) comparison non-micellar sodium caseinate. Reactive lysine and glutamine residues identified using a label-free approach, based identificatio...

A new type of cow’s milk, called A2 has appeared in the dairy aisles supermarkets recent years. Cows’ milk generally contains two major types beta-casein as A1 and types, although there are 13 genetic variants ?-casein: A1, A2, A3, A4, B, C, D, E, F, H1, H2, I G. Studies have shown that ?-casein may be harmful, is a safer choice for human health especially infant nutrition health. The cow repor...

Immobilized metal ion affinity chromatography (IMAC) is now a widely accepted technique for the separation of natural or artificial products that is beginning to find industrial applications. Here, we introduce a novel procedure for the separation of phosphopeptides and phosphorylated proteins by immobilized zinc(II) affinity chromatography. The phosphate-binding site of the affinity gel is an ...

The protein composition of goat milk differs between goat breeds and could present regional trends. The aim of this study was to comparatively analyze the protein composition of goat milk produced by the Alpine and Saanen breeds in northeastern Brazil and to evaluate the antibacterial activity of its protein fractions. SDS-PAGE, 2-DE electrophoresis and RP-HPLC analyses revealed the absence of ...

This study describes the effect of bovine alpha-S1 casein tryptic hydrolysate (CTH) in a representative sample of day-time workers from the general population of Japan with the occurrence of insomnia during the preceding six months. To investigate this issue, 32 subjects, aged between 25 and 40 years, were examined for the subjective sleep quality using the Japanese Pittsburg Sleep Quality Inde...

The production and biochemical properties of cell envelope-associated proteinases from two strains of Streptococcus thermophilus (strains CNRZ 385 and CNRZ 703) were compared. No significant difference in proteinase activity was found for strain CNRZ 385 when cells were grown in skim milk medium and M17 broth. Strain CNRZ 703 exhibited a threefold-higher proteinase activity when cells were grow...

The cell wall proteinase fraction of Streptococcus cremoris HP has been isolated. This preparation did not exhibit any activity due to either specific peptidases known to be located near the outside surface of and in the membrane or intracellular proteolytic enzymes. By using thin-layer chromatography for the detection of relatively small hydrolysis products which remain soluble at pH 4.6, it w...

Plasmids containing wild-type and hybrid proteinase genes were constructed from DNA fragments of the prtP genes of Lactococcus lactis strains Wg2 and SK11. These plasmids were introduced into the plasmid-free strain L. lactis MG1363. The serine proteinases produced by these L. lactis strains were isolated, and their cleavage specificity and rate towards alpha s1- and beta-casein was investigate...