In 1935 one of us described micromethods for ammonia, urea, total nitrogen, uric acid, creatinine, and allantoin (1). These methods are adapted for the analysis of 1 to 2 ml. aliquots of dilute solutions, e.g. 0.1 mg. per cent of ammonia or urea, and for carrying out a large number of analyses simultaneously. These methods all end in a calorimetric measurement. In the present communication meth...

The rate of protein synthesis in the whole body was measured in one fed subject with seven 15N-labelled amino acids (intravenous and oral doses) and two 15N protein mixtures (oral doses only). The rates were determined individually from the urinary excretion of ammonia and total urea over a 12 h experimental period. Except with oral glycine and alanine, the synthesis rates given by ammonia and ...

Aerobic ammonia oxidation is the process that converts ammonium to nitrate and thus links the regeneration of organic nitrogen to fixed nitrogen loss by denitrification. It is performed by a phylogenetically restricted group of Proteobacteria (ammonia-oxidizing bacteria, AOB) that are autotrophic and obligately aerobic. This chapter describes methods for the measurement of ammonia oxidation in ...

Swine wastewater is characterized by high organic matter content, solids, nitrogen (expressed as total ammonia and protein) and heavy metals. This work determines the methanogenic toxicity effect of free ammonia contained in swine wastewater comparing raw swine wastewater (RW) and the liquid fraction of swine wastewater (TW). The values of IC50 (50% of inhibition) obtained for methanogenic bact...

Symbiotic nitrogen recycling enables animals to thrive on nitrogen-poor diets and environments. It traditionally refers to the utilization of animal waste nitrogen by symbiotic micro-organisms to synthesize essential amino acids (EAAs), which are translocated back to the animal host. We applied metabolic modelling and complementary metabolite profiling to investigate nitrogen recycling in the s...

Thiocapsa roseopersicina, Phototrophic Bacteria, Nitrogen Sources, Ammonia Assimilation, Carbon Metabolism Batch cultures of the phototrophic bacterium, Thiocapsa roseopersicina, were grown anaero­ bically in the light either on sulfide with various ammonia concentrations, N 2 or amino acids as nitrogen sources, or on several simple organic substrates in the absence of reduced sulfur com­ pound...

In connection with certain metabolism experiments now in progress in this Institute, the determination of the nitrogen present in the urine of a steer as ammonium compounds and ammonia has become of considerable importance. Recurring peculiarities in the results obtained in some earlier work involving ammonia determinations led to the belief that the chloroform used in the urine as a preservati...

Series of Copper Ruthenium (Cu-Ru) bimetallic catalysts supported on γ-Al2O3 with different metal loading are prepared and investigated for catalytic wet air oxidation of ammonia to nitrogen. The ammonia decomposition activity was studied at three different temperatures i.e. 150oC, 200oC, and 230 oC and it is found that catalytic activity increases with the increase in temperature along with th...

background & aims of the study: nitrogenous compounds could directly create many problems for human's health and the environment. thus, removal of nitrogenous compounds is necessary. among the most important problems, decreasing dissolved oxygen, toxicity for aquatics, eutrophication, converting aerobic medium into anaerobic one and corrosion could be noted. the aim of this study was deter...

A systematic study was carried out to investigate the potential of manganese nitride related materials for ammonia production. A-Mn-N (A= Fe, Co, K, Li) materials were synthesised by nitriding their oxide counterparts at low temperature using NaNH2 as a source of reactive nitrogen. The reactivity of lattice nitrogen was assessed using ammonia synthesis as a model reaction. In the case of Mn3N2,...