Phaseolus vulgaris L. leaves were subjected to various light, CO(2), and O(2) levels and abscisic acid, then given a 10 minute pulse of (14)CO(2) followed by a 5 minute chase with unlabeled CO(2). After the chase period, very little label remained in the ionic fractions (presumed to be mostly carbon reduction and carbon oxidation cycle intermediates and amino acids) except at low CO(2) partial ...

The synthesis, conformational study and inhibitory properties of diverse indolizidine and quinolizidine iminocyclitols are described. The compounds were chemo-enzymatically synthesized by two-step aldol addition and reductive amination reactions. The aldol addition of dihydroxyacetone phosphate (DHAP) to N-Cbz-piperidine carbaldehyde derivatives catalyzed by L-rhamnulose 1-phosphate aldolase fr...

Herein we report the use of the optical properties of citrate-stabilized gold nanoparticles (Au NPs) for estimation of native or denatured conformation content in a mixture of a protein in solution. The UV-vis extinction spectrum of citrate-stabilized Au NPs is known to broaden differently in the presence of native and denatured states of alpha-amylase, bovine serum albumin (BSA) or amyloglucos...

The kaolinite sheets were scrolled by sonication-induced chemical delamination processes into well-developed nanotubes (KNs) which used as substrates for microwave-based ZnO nanoparticles (ZnO/KNs). biological activities of synthetic ZnO/KNs structures, in terms the antioxidant and antidiabetic properties, assessed comparative studies with separated phases KNs well commercially ZnO. substrate r...

Abstract Puccinia triticina ( Pt ) is an important pathogen of wheat. While breeding programmes develop resistant wheat cultivars to mitigate the effects such rust-causing pathogens, emergence new rust races with wider virulence mandates implementation other control strategies. Our study investigated whether acidic pH conditions affected selected Carbohydrate-Active Enzymes (CAZymes) in Pt- ino...

Cultured JV<M?oc spec, from Pe///gero excreted in the light between 1% and 3.4% of fixed "CO, into the medium, <0.8% as glucose. A several-fold increase in the rate of release was caused by addition of carbony! cyanide p-trifluoromethoxyphenylhydrazone (FCCP) in the dark, the major component released being glucose. The amount of glucose excreted in the presence of FCCP exceeded the pool of intr...

Mutations affecting the synthesis of the sporulation amyloglucosidase were isolated in a homothallic strain of Saccharomyces cerevisiae, SCMS7-1. Two were found, both of which were deficient in sporulation at 34 degrees. One, SL484, sporulated to 50% normal levels at 30 degrees but less than 5% at 34 degrees or 22 degrees. The other, SL641, failed to sporulate at any temperature. Both mutants w...

The filamentous fungus Magnaporthe oryzae is the causal agent of rice blast disease. Here we show that glycogen metabolic genes play an important role in plant infection by M. oryzae. Targeted deletion of AGL1 and GPH1, which encode amyloglucosidase and glycogen phosphorylase, respectively, prevented mobilisation of glycogen stores during appressorium development and caused a significant reduct...

Removal of biofilms is difficult. In industrial settings, both the inactivation and removal of biofilms are of huge concern. If only disinfection without the removal of attached biofilms occurs, the inactivated biofilm cells may provide an ideal environment for further adhesion and growth, resulting in a complex matrix. Microbial resistance to biocides and their negative environmental impact ar...