Abstract Recent discoveries of extant and fossilized communities indicate that eukaryotes, including fungi, inhabit energy-poor anoxic environments deep within the fractured igneous crust. This subterranean biosphere may constitute largest fungal habitat on our planet, but knowledge abyssal fungi their syntrophic interactions with prokaryotes concomitant metabolisms is scarce. Here we report fi...

The degradation and fermentation of cellulose filter paper were studied in axenic cultures of 3 species of rumen anaerobic fungi, Neocallimastix frontalis, Piromyces communis and Caecomyces communis, and in cocultures containing 1 of these fungal strains and Eubacterium limosum, a hydrogenotrophic rumen bacterial species. When E limosum was introduced into fungal cultures a slight decrease in f...

Herbivores as well as other vertebrates are not capable of producing cellulases and/or hemicelullases. Instead, a lot of herbivores form symbiotic associations with bacteria, protozoa and fungi which produce these enszymes and therefore are capable of degrading the plant polymers. In return for the relatively constant environment and continuous flow of plant material, the microorganisms of the ...

A method is presented for the specific isolation of genes encoding cellulosome components from anaerobic fungi. The catalytic components of the cellulosome of anaerobic fungi typically contain, besides the catalytic domain, mostly two copies of a 40-amino-acid cysteine-rich, noncatalytic docking domain (NCDD) interspaced by short linkers. Degenerate primers were designed to anneal to the highly...

the aim of this study was to evaluate the effect of substitution of soybean meal with canola meal and measure its effects on rumen anaerobic fungal population in lactation iranian of holstein cows. from d 5 to 56 postpartum, cows were fed diets that were isoenergetic containing soybean meal (sbm; n = 3) or canola meal (cm; n = 3). cows were housed in tie stalls and fed tmr two times a day. comp...