1. A computerized technique is described for the quantitative determination of radiometabolites from incubation studies. 2. Seven steroid substrates have been incubated with human endometrial tissue. The principal radiometabolites were identified and determined after 2hr. incubation without the addition of cofactors and after 4hr. incubation with cofactors. 3. The main products from progesteron...

Adrenocortical glucocorticoid and androgen secretion is stimulated by exercise. Excesses of these hormones in fetuses can cause abnormalities in development. We measured maternal and fetal plasma corticosterone and androstenedione concentrations in Sprague-Dawley rats immediately after maternal exercise in exercise-trained mothers and at rest in sedentary mothers. To do this, we developed a tec...

Despite a relative dearth of information on their effects, supplementation with prohormones has become a popular practice. Unlike synthetic anabolic-androgenic steroids, many of these over-the-counter androgens are produced endogenously by adrenal, gonadal and peripheral steroidogenic pathways as part of the normal sexual and reproductive hormonal milieu. It has been contended that peripheral e...

In an attempt to define the hormonal environment of women with breast cancer, we examined the blood pro duction rates of androstenedione and its contributory role as a prehormone of estrone in a group of 46 postmenopausal women with advanced metastatic breast cancer. Androstenedione blood production rates averaged 1.85 mg/day and were not significantly different from 9 agematched women with oth...

The present study probes putative disruption of hypothalamic control of multihormone outflow in polycystic ovarian syndrome by quantitating the joint synchrony of leptin and LH release in adolescents with this syndrome and eumenorrheic controls. To this end, hyperandrogenemic oligo- or anovulatory patients with polycystic ovarian syndrome (n = 11) and healthy girls (n = 9) underwent overnight b...

10503 Background: Genetic factors that contribute to endogenous estrogen synthesis and postmenopausal breast cancer risk are unknown. We set out test the hypothesis homozygous inheritance of common 1245A→C missense-encoding polymorphism in HSD3B1, which is (8-10%) White populations, functionally adrenal permissive increases aromatase substrate, androstenedione, associated with receptor-positive...

BACKGROUND Since the passage of The Dietary Supplement Health and Education Act in 1994, there has been a flood of new "dietary" supplements promoting anti-aging benefits such as the enhancement of growth hormone or testosterone levels. Androstenediol and androstenedione are such products. This study's purpose was to elucidate the physiological and hormonal effects of 200 mg/d of oral androsten...

The pUfPose of this study was to examine the influence of androstenedione supplementation on plasma testosterone, cortisol, and cholesterol, mean arterial pressure (MAP), body weight, organ weights, and bone mineral density (BMD) in female rats. At age 10 weeks, an initial BMD measure was made. Rats in the andro group (n = 13) received implants designed to deliver 1 J.lg androstenedione per gra...

It has been demonstrated in this laboratory (1) that A4-androstenedione3,17, c&testosterone, and small amount of other unidentified steroids are formed when testosterone is incubated with liver slices. In order to elucidate further the course of the metabolism of these steroids, 2000 mg. of A4-androstenedione-3, 17, the chief metabolite of testosterone (l), has been incubated in lots of 100 mg....

Aromatase activity has been studied in the rainbow trout ovary in a perifusion system. During early vitellogenesis, as in other stages of the annual cycle, the ovaries were capable of aromatizing androstenedione and testosterone. Aromatase activity increased from the postovulatory stages to exogenous vitellogenesis. When highly purified salmon glycoprotein maturational gonadotropin was added to...