Downregulation of the transcription factor AtMYB103 using transgenic technology results in early tapetal degeneration and pollen aberration during anther development in Arabidopsis thaliana. This paper describes the functional analysis of the AtMYB103 gene in three knock-out mutants. Two male sterile mutants, ms188-1 and ms188-2, were generated by ethyl-methane sulfonate (EMS) mutagenesis. A ma...

Cytoplasmic male sterility is a maternally inherited trait in higher plants that prevents the production of functional pollen. Ogura cytoplasmic male sterility in radish (Raphanus sativus) is regulated by the orf138 mitochondrial locus. Male fertility can be restored when orf138 accumulation is suppressed by the nuclear Rfo locus, which consists of three genes putatively encoding highly similar...

RNA editing and cytoplasmic male sterility are two important phenomena in higher plant mitochondria. To determine whether correlations might exist between the two, RNA editing in different tissues of Sorghum bicolor was compared employing reverse transcription-PCR and subsequent sequence analysis. In etiolated shoots, RNA editing of transcripts of plant mitochondrial atp6, atp9, nad3, nad4, and...

Pollen pistil interaction studies were carried out in six sunnhemp genotypes. viability percentage of the genotypes ranged from 0.0 to 93.5% and pollen germination 84.0% at four different floral stages starting bud opening a flower. The viable grains present later three flower for globose type anthers, while two heart shaped anthers. Stigma receptivity was high very during S3 S4 stages. Artific...

Progress in protein labeling by gene fusion with fluorescent protein-encoding genes and microscopic apparatuses, such as a confocal laser-scanning microscope, has enabled time-lapse analysis of meiotic chromosome movement living plant cells. Here, we constructed the pAtDMC1:H2B:GFP introduced it into Arabidopsis thaliana. Whole-genome sequencing confirmed insertion locus between AT3G14830 AT3G1...

The plant hormone jasmonate has been implicated in male fertility in Arabidopsis. Recent studies have identified the enzyme required for a critical step of jasmonate synthesis in anthers and shown that this enzyme really is required for male fertility.

OsSPX1, a rice SPX domain gene, involved in the phosphate (Pi)-sensing mechanism plays an essential role in the Pi-signalling network through interaction with OsPHR2. In this study, we focused on the potential function of OsSPX1 during rice reproductive phase. Based on investigation of OsSPX1 antisense and sense transgenic rice lines in the paddy fields, we discovered that the down-regulation o...

Cytoplasmic male sterile line RC(7) of Chinese cabbage produces mature anthers without pollen. To understand the mechanisms involved, we examined the ultrastructural changes during development of the microspores. Development of microspores was not affected at the early tetrad stage. During the ring-vacuolated period, some large vacuoles appeared in the tapetum cells, making them larger, ext...