introduction: polyclonal antibodies are required to be affinity purified. improved purification methods of polyclonal antibody provide an opportunity to pick the most purified immunoglobulins as a primary or secondary antibody in immunoassays that are included in many vaccine studies. two common techniques for purifying proteins is salt precipitation and chromatography purification. our work fo...

background: dengue is a global arboviral threat to humans; causing 390 million infections per year. the availability of safe and effective tetravalent dengue vaccine is a global requirement to prevent epidemics, morbidity, and mortality associated with it. methods: five experimental groups (6 mice per group) each of 5-week-old balb/c mice were immunized with vaccine and placebo (empty plasmid) ...

background pertussis is a highly communicable, vaccine-preventable respiratory disease; which may circulate even in populations with high vaccination coverage. although frequent, but it is often underestimated as a cause of prolonged cough illness in both children and adults. without boosting, the protection of the childhood vaccination would be minimal after 10 years. the need for adult booste...

shiga toxin (stx) is the principal virulence factor of shigatoxigenic escherichia coli (stec), a food-born pathogen associated disease with uncomplicated diarrhea or the hemolytic-uremic syndrome. in this study, rabbit polyclonal anti recombinant a, b subunits of shiga toxin and holotoxin antisera were raised and employed for immunological purpose. by immunoblotting, these antisera recognized r...

hepatitis b virus (hbv) infection and its sequelae such as cirrhosis and hepatocellular carcinoma has remained a serious public health problem throughout the world. the who strategy for effective control of hbv infection and its complications is mass vaccination of neonates and children within the framework of expanded programme on immunization (epi). vaccination with hepatitis b surface antige...

Background: Human epidermal growth factor receptor 2 (HER2) has a crucial role in several malignancies. The extracellular domain of HER2 (HER2-ECD) has been extensively employed as an important target in passive and active immunotherapy. Isolated recombinant prokaryotic HER2-ECD subdomains were previously found to be ineffective in inducing anti-tumor antibody response. Objective: To employ rec...

  Background: Cigarette smoking has been linked with the suppression of immune responses and increased susceptibility to numerous infections in humans. Tetanus is also a major public health problem in many countries. The aim of the present study was to evaluate the serum levels of anti-tetanus toxin antibodies in c igarette smoking and healthy non-smoking people .   Materials and M...

background and purpose: multiple sclerosis (ms) is believed to be an immune-mediated disorder that develops from an interaction of the individual's genetic and as yet unidentified environmental causes. the prevalence of auto-antibodies in multiple sclerosis patients and their clinical associations vary in various studies. the aim of this study was to determine serum auto antibodies in multiple ...

long-term protection against hepatitis b virus (hbv) is dependent on persistence of anti-hbs antibodies and/or strong immunological memory. in this study we evaluated the persistence of anti-hbs antibodies in healthy iranian children 5 years after primary vaccination and the response to a booster dose using recombinant hepatitis b vaccine. totally, 81 children who had received primary course of...

background: inflammatory bowel disease unclassified (ibdu) is considered to be an aberrant immune response with loss of tolerance to many antigens. objective: this paper tries to address whether there is any value to test for auto-antibodies in such patients. methods: 60 patients with inflammatory bowel disease unclassified participated in the study. auto-antibodies to nuclear antigen, intestin...