PURPOSE Monoclonal antibodies or specific antibodies are now an essential tool of biomedical research and are of great commercial and medical value. The purpose of this study was to produce large scale of monoclonal antibody against CD34 in order to diagnostic application in leukemia and purification of human hematopoietic stem/progenitor cells. METHODS For large scale production of monoclona...

Development of a stool antigen immunoassay to detect Helicobacter pylori infection requires monoclonal antibody against the specific antigen. Alkylhydroperoxide reductase (AhpC) of Helicobacter pylori has been described as a specific and unique enzyme for H. pylori and therefore, both H. pylori AhpC and Anti-AhpC could be useful in the development of serologic and stool antigen tests, for detec...

The increasing demand for monoclonal antibodies (MAb) as biopharmaceuticals has promoted the development of cell cultures with increased expression levels. As a consequence, the demand for more efficient purification processes has increased. This application note describes a two-step process for MAb purification based on MabSelect SuReTM and CaptoTM adhere, a strong multimodal anion exchanger. ...

background: equine chorionic gonadotropin (ecg) is commonly used in association with a progestagen treatment to synchronize estrus of goats and ewes during breeding and non-breeding seasons. classical purification of the ecg from serum includes ph fractionation with metaphosphoric acid two ethanol precipitation steps as well as dialysis followed by fixed-bed chromatography. objectives: the aim ...

Antibodies play an essential role in modern medicine for diagnostic and therapeutic applications. Even though the production of antibodies is fastest growing pharmaceutical industry area, cost remains high, which limits access to antibody-based both developing developed countries. The bottleneck major factor purification antibody. Here, a proof-of-concept presented antibody extraction using fer...

Plant-based expression systems provide an alternative biomanufacturing platform for recombinant proteins (Matoba et al., 2011). In particular, plant virus-based vectors can overexpress proteins within days in the leaf tissue of Nicotiana benthamiana (N. benthamiana). To overcome the issues of genetic instability and limited infectivity of recombinant viruses, Agrobacterium-mediated delivery of ...