The common acute lymphoblastic leukemia antigen (CALLA), as defined by J-5 murine monoclonal antibodies, was detected on renal tubular and glomerular cells from fetal and adult donors by an indirect immunoperoxidase technique. CALLA could also be detected on epithelial cells of the fetal small intestine and on myoepithelial cells of adult breast but not on myoepithelial cells of the salivary gl...

The expression of common acute lymphoblastic leukemia antigen (CALLA) on a human neuroblastoma cell line, SJ-N-CG, was demonstrated by indirect membrane immunofluorescence, complement-dependent cytotoxicity, and quantitative absorption, using two monoclonal antibodies (J-5 and BA-3) directed against CALLA. Immunoprecipitation of solubilized 125I-labeled membrane proteins from SJ-N-CG cells with...

Several studies have demonstrated roles for eosinophils during innate and adaptive immune responses to helminth infections. However, evidence that eosinophils are capable of initiating an immune response to parasite antigens is lacking. The goal of the present in vitro study was to investigate the potential of eosinophils to serve as antigen-presenting cells (APC) and initiate an immune respons...

fast enzyme linked immunosorbent assay (fast-elisa) was compared with the standard elisa for the diagnosis of human hydatidosis. seventy serum samples including 30 from hydatidosis patients (surgically confirmed), healthy control individuals not infected with any parasitic diseases (n=/20) and from others with different parasitic infections including, toxocariosis (n=5), fasciolosis (n=5), tric...

Drs. J. Jaradat and J. Zawistowski have recently reported three monoclonal antibodies (MAbs) against the O antigens of Salmonella typhimurium lipopolysaccharide (LPS) (9). These antibodies were characterized to bind O antigen 5 (or O:5), which corresponds immunochemically to O-acetyl abequose (7). Other laboratories have also developed MAbs against O antigens of S. typhimurium (summarized in Ta...

The human hepatocellular carcinoma cell line PLC/PRF/5, which synthesizes and secretes hepatitis B surface antigen, was grown under optimal conditions in tissue culture, using Eagle's minimal essential medium supplemented with 10% fetal bovine serum and 10(-11) M triiodothyronine on collagen rafts. Injection s.c. of the PLC/PRF/5 cell line into athymic BALB/c nude mice resulted in the growth of...

background: cervical cancer is one of the most common types of women’s reproductive cancers. a major risk factor for cervical cancer is exposure to a group of common sexually transmitted viruses called human papillomavirus  (hpv). the over expression of hpv 16 i.e., e6 antigen in the tumors of suspected cervical cancer tissues. however e-6 antigen has hyaluronan binding protein site. methods: t...

A novel tumour associated antigen, 22-1-1, has been recently described in association with a cervical adenocarcinoma cell line. The aims of this paper were to study the tissue distribution of this antigen in sections of gynaecological cancer specimens and to compare it with negative controls. Six cases of cervical cancers, 5 cases of endometrial cancers, 4 cases of ovarian cancers and 5 cases e...

Detection of FMDV type O antigen using Immunohistochemistry (IHC) technique was performed on formalin-fixed, paraffin-embedded (FFPE) tissues obtained from cattle originally intradermolingual inoculated with FMDV type O strain O Udornthani/87, FMDV type A strain A Lopburi/2012 and FMDV type Asia-1 strain Asia-1 Nakornpathom/85 to consider the cross reaction. The number of animals used for these...

detection of bovine leukemia virus antigens can induces humoral immunity in cow were evaluated. fifteen lymph node from infected cows that had positive results in agid and elisa tests for blv and have clinical signs of lymphosarcoma, in addition five lymph node from apparently healthy cows that had negative results in serological tests. after preparation of extract of lymph nodes, all of the sa...