We have characterized chicken gizzard smooth muscle Ca2+/calmodulin-dependent protein kinase II (CaM-PKII) with particular focus on its autophosphorylation. The autophosphorylation of smooth muscle CaMPKII produced a partially constitutively active enzyme, as occurs with the alpha- and beta-isoforms of this enzyme, but the autophosphorylation kinetics were significantly slower. Phosphorylation ...

The imbalance between nitric oxide (NO) and reactive oxygen species (ROS) production appears to be a common feature of experimental and human hypertension. Previously, different antioxidants and/or scavengers of oxygen free radicals were shown to activate nitric oxide synthase (NO synthase, NOS) and to increase the expression of both endothelial and neuronal NO synthase isoforms leading to bloo...

PURPOSE The goal of this study was to identify potential protein markers in lung adenocarcinomas. EXPERIMENTAL DESIGN A series of 93 lung adenocarcinomas (64 stage I and 29 stage III) and 10 uninvolved lung samples were examined for quantitative differences in protein expression using two-dimensional PAGE. Candidate proteins were identified using matrix-assisted laser desorption/ionization ma...

Dairy cattle are at the greatest risk of developing diseases around time calving because compromised immune responses and occurrence oxidative stress. Both development immunity stress influenced directly or indirectly by metabolism polyunsaturated fatty acids (PUFA) fat-soluble vitamins. The cytochrome P450 (CYP450) family enzymes is central to both classes these compounds, but date, importance...

Preterm infants exposed to high concentration oxygen are prone to develop hyperoxic lung damage, which is an importan t underly ing cause of bronchopulmonary dysplasia (BPD).1 Although the causative agent for BPD has not been conclusively identified, hyperoxia-induced lung injury i s bel ieved to be a major factor. Data from both cellu lar and whole animal models suggest that hyperoxic lung dam...

The Omega class of glutathione transferases (GSTs) forms a distinct within the cytosolic GST superfamily because most them possess catalytic cysteine residue. human 1 isoform was first characterized twenty years ago, but it took work to clarify roles isoforms. Concerning kingdom fungi, little is known about cellular functions (GSTOs), although they are widely represented in some these organisms...

Comprehensive comparison of antioxidant activity bioactive peptides produced from fish, poultry and shrimp wastes using Flavourzyme enzyme

Previously, we have demonstrated that chronic consumption of a high-fat, high-refined sugar (HFS) diet results in metabolic syndrome which is marked by obesity, insulin resistance, hyperlipidemia, and hypertension in Fischer rats. Metabolic syndrome in this model is associated with oxidative stress, avid nitric oxide (NO) inactivation by reactive oxygen species (ROS), diminished NO bioavailabil...

We identified five Na+/K+-ATPase alpha-isoforms in rainbow trout and characterized their expression pattern in gills following seawater transfer. Three of these isoforms were closely related to other vertebrate alpha1 isoforms (designated alpha1a, alpha1b and alpha1c), one isoform was closely related to alpha2 isoforms (designated alpha2) and the fifth was closely related to alpha3 isoforms (de...