Antisense regulation of IS10 transposase synthesis is mediated by a small RNA molecule, RNA-OUT which is complementary to the 5' region of the IS10 transposase mRNA, RNA-IN. Pairing between the two species in vivo prevents initiation of RNA-IN translation by steric occlusion of the ribosome binding site. The goal of this work is to develop a mathematical basis for antisense repression in vivo. ...

Antisense therapy involves the use of antisense oligonucleotides for altering targeted gene function. However, the low efficiency of cell delivery of antisense oligonucleotides has limited the efficacy of antisense therapeutic approaches. RNA-based antisense or ribozyme oligonucleotides can be either synthesized endogenously (e.g., by a viral vector) or delivered exogenously. However, there is ...

This month's installment of Generally Physiological focuses on endogenous regulation of K + channels by anti-sense, K + channel targeting by anti-bodies, and the effects of gastric by pass surgery on metabolism. A decrease in the abundance of voltage dependent K + channels in dorsal root ganglion (DRG) neurons can result in enhanced excitability and aberrant firing, contributing to neu-ropathic...

This communication describes a two unit antisense RNA cassette system for use in gene silencing. Cassettes consist of a recognition unit and an inhibitory unit which are transcribed into a single RNA that carries sequences of non-contiguous complementarity to the chosen target RNA. The recognition unit is designed as a stem-loop for rapid formation of long- lived binding intermediates with targ...

tissue is accomplished by removal of keratinocytes harboring HPV. These are the conventional approaches for treatment of cervical cancer. Prophylactic vaccines are available and should be administered prior to viral exposure. Initiatives for vaccination have been undertaken by the governments and also non-governmental agencies. Attempts are being made to develop therapeutic vaccines that would ...

The HIV-1 genome consists of two identical RNA molecules non-covalently linked by their 5' unstranslatable regions (5' UTR). The high level of sequence and structural conservation of this region correlates with its important functional involvement in the viral cycle, making it an attractive target for antiviral treatments based on antisense technology. Ten unmodified DNA antisense oligonucleoti...

Human papillomavirus type 16 (HPV-16) transcription was analysed in one squamous cervical carcinoma by cDNA cloning and DNA sequencing, and in eight additional squamous cervical carcinomas and 11 precancerous lesions by RNA-RNA in situ hybridization. The nucleotide sequences of the cDNA clones revealed structures of early HPV-16 mRNAs (E6*-E7-E1 E4-E5) in agreement with data reported for other ...

To investigate the effect of long non coding RNA MAGI2-antisense 3 on proliferation, invasion and migration cervical cancer cells underlying mechanism. SiHa were divided into plasmid cloning DNA group, DNA-MAGI2-antisense anti-microRNA-negative control anti-microRNA-31-5p 3+microRNAnegative group 3+microRNA-31-5p group; quantitative reverse transcription polymerase chain reaction was used to de...

BACKGROUND AND AIMS The arabinogalactan protein (AGP) gene family is involved in plant reproduction. However, little is known about the function of individual AGP genes in pollen development and pollen tube growth. In this study, Brassica campestris male fertility 8 (BcMF8), a putative AGP-encoding gene previously found to be pollen specific in Chinese cabbage (B. campestris ssp. chinensis), wa...