The results of this study indicate that very low density lipoprotein (VLDL) from the plasma of fasting normolipidemic or slightly hypertriglyceridemic subjects contains two apo B species. In SDS gel electrophoresis, the VLDL shows the presence of a major band corresponding to low density lipoprotein (LDL) apo B (apo B-100) and a minor band with the appropriate mobility of the lymph chylomicron ...

APO-1 is a cell surface molecule that induces apoptosis when ligated with the monoclonal antibody anti-APO-1. Expression of APO-1 and response to anti-APO-1 was investigated in a number of Epstein-Barr virus (EBV)-positive and -negative Burkitt lymphoma (BL) cell lines, in EBV-immortalized lymphoblastoid cell lines, and in cells from fresh BL biopsies. APO-1 was not expressed in EBV-negative ce...

Bacterial endotoxin is a potent inducer of the serum amyloid protein (apo-SAA), a high density lipoprotein (HDL) apoprotein. In a study of the induction of apo-SAA and the structure of apo-SAA-rich lipoprotein particles in mice, we have observed that, following intraperitoneal administration of Salmonella typhosa lipopolysaccharide (50 micrograms), plasma apo-SAA levels rose from base-line leve...

BACKGROUND The apo B/apo A-I ratio represents the balance between atherogenic particles, rich in apo B, and the antiatherogenic ones, apo A-I rich. This study investigated the association between atherosclerotic diseases in different anatomical sites and apo B/apo A-I ratio. METHODS Lipids, lipoproteins, and apolipoproteins A-I and B were assessed in 30 subjects with coronary artery disease (...

Human apolipoprotein (apo)-B mRNA undergoes a novel tissue specific editing reaction which replaces a genomically templated cytidine with uridine. This substitution converts codon 2153 from glutamine (CAA) in apo-B100 mRNA to a stop codon (UAA) in apo-B48 mRNA. This novel RNA editing process is responsible for the generation of hepatic apo-B100 and intestinal apo-B48. We have established the fo...

Abstract We report the G protein-first mechanism for activation of protein-coupled receptors (GPCR) three closest subtypes opioid (OR), ?OR, ?OR and ?OR. find that they couple to inactive Gi protein-bound guanosine diphosphate (GDP) prior agonist binding. The protein forms anchors intracellular loops apo-?OR, apo-?OR apo-?OR, inducing opening cytoplasmic region form a pre-activated state holds ...

Aluminum phosphates (APO-5s) were synthesized and evaluated as heterogeneous catalysts for the valorization of furfural to furfuryl acetals (FAs) alcohol ethers (FAEs). Weakly acidic APO-5 (Al/P = 1) exhibited excellent catalytic performance with 96 % FA yield in ethylene glycol, analysis acetalization reaction supported that adsorption catalyst sites co-catalyzed reaction. In addition, was sta...

BACKGROUND A reliable method for plasma would be useful to investigate the role of apolipoprotein (apo) AIV when associated with apo B-containing or triglyceride-rich lipoproteins. METHOD We used a sandwich ELISA to quantify lipoprotein B:AIV particles (Lp B:AIVf; lipoproteins containing at least apo B and apo AIV) in plasma. The method used microtiter plates coated with purified anti-apo B i...

After in vitro incubation with radiolabeled amino acids, extracts of various chicken tissues were reacted with antiserum against apolipoprotein AI (apo-AI) of plasma high density lipoprotein. Radiolabeled apo-AI was found in liver and intestine as well as in the kidney and a variety of peripheral tissues including aorta, peripheral arteries, veins, and skeletal muscle. The immunoreactive apo-AI...

The effects of dexamethasone (a synthetic glucocorticoid) and insulin on the secretion of very-low-density lipoprotein (VLDL) and high-density lipoprotein (HDL) were investigated. Rat hepatocytes in monolayer culture were preincubated for 15 h in the presence or absence of combinations of 100 nM-dexamethasone and 2 nM-, 10 nM- or 50 nM-insulin. Dexamethasone increased [3H]oleate incorporation i...