The Fc glycosylation of therapeutic antibodies is crucial for their effector functions and their behavior in pharmacokinetics and pharmacodynamics. To monitor the Fc glycosylation in bioprocess development and characterization,high-throughput techniques for glycosylation analysis are needed. Here, we describe the development of a largely automated high-throughput glycosylation profiling method ...

The Newark-APTS established a high-resolution framework for the Late Triassic and Early Jurassic. Palaeomagnetic polarity correlations to marine sections show that stage-level correlations of continental sequences were off by as much as 10 million years. New U–Pb ages show the new correlations and the Newark basin astrochronology to be accurate. Correlation of Newark-APTS to the Chinle Formatio...

High-throughput methods for oligosaccharide analysis are required when searching for glycan-based biomarkers. Next to mass spectrometry-based methods, which allow fast and reproducible analysis of such compounds, further separation-based techniques are needed, which allow for quantitative analysis. Here, an optimized sample preparation method for N-glycan-profiling by multiplexed capillary gel ...

Developing a detailed threat profile, provides organizations with a clear illustration of the threats that they face, and enables them to implement a proactive incident management program that focuses on the threat component of risk. Organizations are facing new types of advanced persistent threat (APT) scenarios that existing risk management programs are not able to evaluate completely and inc...

The molecular deposition of starch extracted from normal plants and transgenically modified potato lines was investigated using a combination of light microscopy, environmental scanning electron microscopy (ESEM) and confocal laser scanning microscopy (CLSM). ESEM permitted the detailed (10 nm) topographical analysis of starch granules in their hydrated state. CLSM could reveal internal molar d...

An advanced persistent threat (also known as APT) is a deliberately slow-moving cyberattack that is applied to quietly compromise interconnected information systems without revealing itself. APTs often use a variety of attack methods to get unauthorized system access initially and then gradually spread throughout the network. In contrast to traditional attacks, they are not used to interrupt se...

Recently, magnetic nanoparticles of iron oxide (Fe3O4, c-Fe2O3) have shown an increasing number of applications in the field of biomedicine, but some questions have been raised about the potential impact of these nanoparticles on the environment and human health. In this work, the three types of magnetic nanoparticles (DMSAFe2O3, APTS-Fe2O3, and GLU-Fe2O3) with the same crystal structure, magne...

A series of pyrene photoacids is used to investigate excited-state proton transfer with time-dependent pump-probe spectroscopy. The deprotonation dynamics of a cationic photoacid, 8-aminopyrene-1,3,6-trisulfonic acid trisodium salt (APTS), shows single exponential dynamics( approximately 30 ps) in water. This is in contrast to what is observed for the neutral photoacids 8-hydroxypyrene-1,3,6-tr...

Targeted attacks on IT systems are a rising threat against the confidentiality of sensitive data and the availability of critical systems. With the emergence of Advanced Persistent Threats (APTs), it has become more important than ever to fully understand the particulars of such attacks. Grammar inference offers a powerful foundation for the automated extraction of behavioral patterns from sequ...

BACKGROUND Transplantation of allogeneic adipose-derived regenerative cells (ADRCs) is a promising treatment modality for severe ischemic diseases. However, minimal information is available on the in vivo effects, fate, and migration of ADRCs, as well as the mechanisms of their therapeutic angiogenesis. MATERIALS AND METHODS In this study, green fluorescent protein-expressing ADRCs (GFP-ADRCs...