A liquid medium containing wheat bran, salts and a source of inducer (pectin) was found to be suitable for the production of exoand endo-polygalacturonases by Aspergillus oryzae CCT3940. Induction of polygalacturonases by purified pectin was significantly higher than when rinds of citrus fruits were used as inducer. A. oryzae growth was favoured by pH close to 4, although a drop of pH to around...

Cyclopiazonic acid (CPA) is an indole-tetramic acid neurotoxin produced by some of the same strains of A. flavus that produce aflatoxins and by some Aspergillus oryzae strains. Despite its discovery 40 years ago, few reviews of its toxicity and biosynthesis have been reported. This review examines what is currently known about the toxicity of CPA to animals and humans, both by itself or in comb...

Cutinase thermostability is important so that the enzymes can function above the glass transition of what are often rigid polymer substrates. A detailed thermal inactivation analysis was performed for two well-characterized cutinases, Aspergillus oryzae Cutinase (AoC) and Thiellavia terrestris Cutinase (TtC). Both AoC and TtC are prone to thermal aggregation upon unfolding at high temperature, ...

Q. ZUNE*, DELEPIERRE A*; D. TOYE**, P. J. PUNT*** F. DELVIGNE**** *Unit of BioIndustry, Gembloux AgroBioTech (ULg). Passage des Déportés, 2; 5030 Gembloux. **Laboratory of Chemical Engineering, ULg. Allée de la Chimie, 3/6c. B-4000 Liege. ***Wageningen Centre for Food Sciences, P.O. Box 557, 6700 AN Wageningen (The Netherlands). ****Unit of BioIndustry, Gembloux AgroBioTech (ULg). F.R.S-F.N.R.S...

The suitability of protein and glucosamine measurements of cell components for biomass estimation of fungi Aspergillus niger S,4, a mangrove isolate, and Aspergillus oryzae NCIM 1212 was evaluated using wheat bran as substrate in solid-state fermentation (SSF). Both direct as well as indirect methods were used. The protein and glucosamine contents and direct weight of the fungi were determined ...

From an industrial point of view, the use microorganisms as a wastewater bioremediation practice represents sustainable and economic alternative for conventional treatments. In this work, we investigated starch paper mill (PMW) with Aspergillus oryzae. This amylase-producing fungus was tested in submerged fermentation technology (SmF) solid-state (SSF) on rice hulls. The tests were conducted to...

The nitrate reductase gene (niaD) is the most frequently utilized as a selectable marker for homologous integration at the niaD locus of Aspergillus oryzae. In this study we developed a method for curing of the niaD-based plasmid integrated on the A. oryzae genome. Positive selection using a modified chlorate medium containing leucine as a nitrogen source enabled efficient isolation of the stra...

Aspergillus oryzae, an important filamentous fungus used in food fermentation and the enzyme industry, has been shown through genome sequencing and various other tools to have prominent features in its genomic composition. However, the functional complexity of the A. oryzae transcriptome has not yet been fully elucidated. Here, we applied direct high-throughput paired-end RNA-sequencing (RNA-Se...

Aspergillus flavus is the main etiological agent for aflatoxin contamination of crops. Its close relative, A. oryzae, does not produce aflatoxins and has been widely used to produce fermented foods. We compared the phylogeny of A. oryzae isolates and L- and S-type sclerotial isolates of A. flavus using single nucleotide polymorphisms in the omtA gene in the aflatoxin biosynthesis gene cluster a...

In the production of industrial enzymes, filamentous fungi are attractive as cell factories because they can secrete large amounts of protein into the medium. Many important industrial enzymes such as amylases, lipases and cellulase are produced by fermentation of filamentous fungi. Aspergillus oryzae is well-known filamentous producer of alpha amylase and has GRAS status. In the present study ...