Background. Daclatasvir (DCV) is a potent hepatitis C virus (HCV) NS5A replication complex inhibitor with pangenotypic (GT 1-6) activity in vitro. Asunaprevir (ASV) is a selective NS3 protease inhibitor with in vitro activity against GT 1, 4, 5 and 6. These two direct-acting antivirals (DAA) are in phase 3 development and regulatory review as a dual-DAA regimen (DCV + ASV) for the treatment of ...

AsIII uptake in living cells is through aquaglyceroporin transporters, but it is unknown in arsenic-hyperaccumulator Pteris vittata. We investigated the effects of AsIII analogs glycerol and antimonite (SbIII) at 0-100 mM and aquaporin inhibitor AgNO(3) at 0-0.1 mM on the uptake of 0.1 mM AsIII or AsV by P. vittata over 1-2 h. Glycerol or SbIII didn't impact AsIII or AsV uptake by P. vittata (p...

Asunaprevir (ASV), an isoquinoline-based competitive inhibitor targeting the hepatitis C virus (HCV) NS3/4A protease, is very potent in vivo. However, the potency is significantly compromised by the drug resistance mutations R155K and D168A. In this study three crystal structures of ASV and an analogue were determined to analyze the structural basis of drug resistance susceptibility. These stru...

During the last years a growing interest in automatic speaker verification (ASV) systems developed. Recent ASV systems still produce two kinds of errors to a considerable extent: false acceptances and false rejections of speakers. The systems generally react extremely sensitive to intra-speaker variability of voice and speaking style, caused for example by different psychological states of the ...

Usually, we need a large number of testing samples to evaluate the performance of automatic speaker verification (ASV) systems. The equal error rate (EER) is a common measure for this purpose. It is derived according to the threshold determined by finding the verification score when the false rejection rate (FRR) equals to the false acceptance rate (FAR). In this paper, a method of model-based ...

It is well known that automatic speaker verification (ASV) systems can be vulnerable to spoofing. The community has responded to the threat by developing dedicated countermeasures aimed at detecting spoofing attacks. Progress in this area has accelerated over recent years, partly as a result of the first standard evaluation, ASVspoof 2015, which focused on spoofing detection in isolation from A...

PURPOSE This study was a retrospective review of 11 limbs of 10 patients with popliteal artery entrapment syndrome (PAES) treated surgically in a 20-yr period. METHODS The patients were aged 34.7 +/- 17.4 SEM yr. Arteriographic findings varied, showing medial deviation in two limbs, poststenotic dilatation in four limbs and occlusion of the popliteal artery in five limbs. In addition, compute...

Any biometric recognizer is vulnerable to direct spoofing attacks and automatic speaker verification (ASV) is no exception; replay, synthesis and conversion attacks all provoke false acceptances unless countermeasures are used. We focus on voice conversion (VC) attacks. Most existing countermeasures use full knowledge of a particular VC system to detect spoofing. We study a potentially more uni...

Two techniques were used to search for the polypeptide encoded by the avian sarcoma virus (ASV) src gene. First, antiserum from rabbits bearing ASV-induced fibrosarcomas was used to immunoprecipitate a transformation-specific antigen from ASV-transformed chick embryo fibroblasts. This antigen has an apparent molecular weight (Mr) of 60,000. Second, the 3' one-third of the ASV genome, selected b...

INTRODUCTION Daclatasvir and Asunaprevir (DCV/ASV) have recently been approved for the treatment of chronic hepatitis C virus infection. In association, they are more effective and safer than previous available treatments, but more expensive. It is unclear if paying for the additional costs is an efficient strategy considering limited resources. METHODS A Markov model was built to estimate th...