Background: Neuro-ichthyotic disorder characterized by ichthyosis, spastic quadriplegia and mental retardation is a rare condition, caused by recessive mutations in the gene ELOVL4, encoding elongase-4 protein. The objective of the study was to search for sequence variants in the gene ELOVL4 in three affected individuals of a consanguineous Pakistani family exhibiting features of neuroichthyoti...

Thirty-one strains of Acinetobacter species, including type strains of the 18 genomic species and 13 clinical isolates, were compared by amplified ribosomal DNA restriction analysis (ARDRA), random amplified polymorphic DNA analysis (RAPD), and amplified fragment length polymorphism (AFLP) fingerprinting. ARDRA, performed with five different enzymes, showed low discriminatory power for differen...

In crude extracts of plant tissue, the M(r) = 100,000 proton-pumping ATPase constitutes less than 0.01% of the total cell protein. A large-scale purification procedure is described that has been used to obtain extensive protein sequence information from this enzyme. Plasma membrane vesicles enriched in ATPase activity were obtained from extracts of oat roots by routine differential and density ...

Source/description: Horse genomic DNA was digested with the restriction enzyme MboI, size selected by gel electrophoresis for fragments between 200 and 1200 bp, and ligated into the BamHI site of the M13 phage vector. Clones containing a potential microsatellite were identified by screening the library with [P] 5¢ end-labelled oligo [dCA]16 and oligo [dGT]16 probes. The DNA was isolated from po...

The data on ten SNP markers of Myostatin gene (GDF-8) was generated on nine breeds/populations of indigenous poultry and Red Jungle Fowl (RJF). The SNPs were five in promoter region, one each in intron 1 and 2 and three in exon 1. The data was analyzed to find out the genetic relationship among the indigenous chicken populations. PCR-RFLP was carried out to genotype the populations at seven SNP...

The polymorphism of a TTC/TTTC microsatellite in the promoter sequence of the elongation factor 3 gene of Candida albicans was investigated by PCR. One primer was fluorescein labeled, and PCR signals were read with an automatic sequencer. Twenty-nine reference strains and 31 independent clinical isolates were studied. Eleven different alleles were identified, giving 16 different profiles among ...

AIM To confirm the presence of recombination, full-length hepatitis B virus (HBV) from chronic patients was sequenced and analyzed. METHODS Full-length HBV genomes from 12 patients were amplified and sequenced in an automated sequencer. Phylogenetic analysis was carried out on full-length, Core and preS2/Surface regions using MEGA software. SimPlot Boot Scanning and amino acid sequence analys...

This paper presents a novel end-to-end approach to program repair based on sequence-to-sequence learning. We devise, implement, and evaluate system, called SequenceR, for fixing bugs learning source code. uses the copy mechanism overcome unlimited vocabulary problem that occurs with big Our system is data-driven; we train it 35,578 samples, carefully curated from commits open-source repositorie...

The genetic diversity in the Kilakarsal sheep population was analysed using 25 microsatellite markers by multiplex PCR in five panels and automated DNA sequencer. The number of alleles ranged from 3 to 13 with a mean of 7.6 across loci. The mean effective number of alleles was 3.88. The size and frequencies of the alleles ranged from 80 to 278 bp and from 0.0100 to 0.8000, respectively. The PIC...