OBJECTIVES The aims of this study were to determine the prevalence of extended-spectrum beta-lactamases (ESBLs) in AmpC-carrying Enterobacter spp. in a tertiary care university hospital in Vienna, Austria, and to implement a cost-effective strategy to detect ESBLs in this particular genus on a routine basis. METHODS Clinical Enterobacter isolates (n=208) were investigated by means of (i) an i...

Epidemiologic surveillance study was conducted in southern Japan to determine the antimicrobial resistance phenotypes and characterize the β-lactamase genes and the plasmids harboring these genes in Salmonella enterica serovar Infantis (S. Infantis) isolates from broilers. Between January, 2007 and December, 2008, a total of 1,472 fecal samples were collected and examined at the Laboratory of V...

BACKGROUND Proteus species cause a variety of community- and hospital-acquired illnesses. Synthesis of β-lactamases is the predominant mechanism for resistance to β-lactam antibiotics. Among the β-lactamases, extended spectrum β-lactamases (ESBLs) and AmpC β-lactamases are the most common. AIM The objective of this study was to determine the occurrence of ESBL and AmpC β-lactamases in Proteus...

TEM- or SHV-type extended-spectrum beta-lactamases(ESBLs) are of clinical concern in Europe and the United States, whereas bacterial strains producing such types of ESBLs had not been reported in Japan for many years. Toho-1, a different type class A ESBL, has been reported in 1995, in which any prototypical enzyme has not been identified so far. At present Toho-1 is the major ESBL in Japan, ho...

background  and  objectives: pseudomonas  aeruginosa is responsible for devastating nosocomial infections among severely burn patients. class c of cephalosporinase (ampc-β-lactamases) is important cause of multiple β-lactam resistance in p. aeruginosa . the aim of this study was to detect the ampc-β-lactamases producing isolates among carbapenem resistant p.  aeruginosa isolated from burn patie...

Cefoxitin-resistant Escherichia coli (n = 109) and Klebsiella pneumoniae (n = 16) isolates collected from patients in India in 2009 to 2010 were screened for bla(ampC) families and mobilizing elements (ISEcp1, IS26, ISCR1, and sul-1-type class 1 integrons) and their association with bla(ampC) and for the occurrence of class A beta-lactamases (BLs) (CTX-M, TEM, and SHV). The concurrent occurrenc...

OBJECTIVES To identify the mechanism(s) underlying cefotaxime resistance in 118 of 21,641 (0.55%) non-typhoidal Salmonella enterica isolates collected from humans throughout England and Wales from January 2010 to September 2012. METHODS Non-duplicate isolates (n = 118) resistant to cefotaxime (MICs >1 mg/L) were screened by PCR for genes encoding CTX-M extended-spectrum β-lactamases (ESBLs) a...

To the Editor: Typhoid fever, caused by Salmonella enterica sero-var Typhi, is a serious form of enteric fever. In 2000, the worldwide number of typhoid cases was estimated to be >21,000,000, and there were >200,000 deaths from this disease (1). Ciprofl oxacin is the fi rst-line drug of choice for treatment of patients with typhoid fever, but there has been an increase in strains resistant to c...

BACKGROUND AmpC type β-lactamases are commonly isolated from extended-spectrum Cephalosporin-resistant Gram-negative bacteria. Also, resistance appeared in bacterial species not naturally producing AmpC enzymes. Therefore, a standard test for the detection of the plasmid-mediated AmpC enzyme and new breakpoints for extended spectrum Cephalosporins are urgently necessary. OBJECTIVES To detect ...

Current study was conducted to determine prevalence and antimicrobial susceptibility patterns, ESBL, AmpC and carbapenemase- producers among clinical isolates of Enterobacteriaceae. Three hundred and seven non-duplicative clinical isolates were collected from hospitalized patients in five medical centers in Azerbaijan, Iran. Initial screening for β-lactamase production was performed using disc ...