BACKGROUND Over 700 bacterial species reside in human oral cavity, many of which are associated with local or distant site infections. Extensive characterization of the oral microbiome depends on the technologies used to determine the presence and proportions of specific bacterial species in various oral sites. OBJECTIVE The objective of this study was to compare the microbial composition of ...

Genomes of all priority bacterial pathogens for biodefense purposes and more than one hundred other bacteria have been sequenced. In addition four hundred bacterial genome-sequencing projects are in progress. These achievements provide new possibilities for reliable identification of bacteria on a molecular level by retrieving their genomic information. We present a mass spectrometric approach ...

Timely classification and identification of bacteria is of vital importance in many areas of public health. We present a mass spectrometry (MS)-based proteomics approach for bacterial classification. In this method, a bacterial proteome database is derived from all potential protein coding open reading frames (ORFs) found in 170 fully sequenced bacterial genomes. Amino acid sequences of tryptic...

The genomic sequences of bacterial pathogens and of the host species they infect have greatly increased the understanding of host-pathogen interactions. Sequences of bacterial genomes have led to the identification of virulence factors through the use of bioinformatics, targeted mutant library construction, screening approaches combining transposon mutagenesis and microarray technology, and thr...

....................................................................................................................... 6 LIST OF ORIGINAL PUBLICATIONS ......................................................................... 8 ABBREVIATIONS ............................................................................................................ 9 INTRODUCTION ..................................

Applications of bacterial endophytes and their advanced identification methodologiesR. Renugadevi, M. P. Ayyappadas, V. Subha Priya, Flory Shobana, K. Vivekanandhan

Applications of bacterial endophytes and their advanced identification methodologiesR. Renugadevi, M. P. Ayyappadas, V. Subha Priya, Flory Shobana, K. Vivekanandhan

Using full 16S ribosomal RNA (rRNA) gene sequencing as the gold standard, 20 non-duplicating anaerobic Gram positive bacilli isolated from blood cultures were analysed by the MicroSeq 500 16S rDNA bacterial identification system. The MicroSeq system successfully identified 13 of the 20 isolates. Four and three isolates were misidentified at the genus and species level, respectively. Although th...

The in situ detection and identification of environmental microorganisms is important for general microbial ecology research. In addition, the rapid detection of microbial contamination in food-processing plants is essential. The current paper proposes a method for the rapid detection and identification of microorganisms using spectral imaging. Photosynthetic and non-photosynthetic bacterial co...

Bacteremia continues to result in significant morbidity and mortality, particularly in patients who are immunocompromised. Currently, patients with suspected bacteremia are empirically administered broad-spectrum antibiotics, as definitive diagnosis relies upon the use of blood cultures, which impose significant delays in and limitations to pathogen identification. To address the limitations of...