Previous studies from this laboratory have shown that a cytidine to uridine base change at the wobble position of the anticodon of Escherichia cdi formylmethionine tRNA (C&j or at one of the cytidine residues of the 3’-terminal C-C-A sequence (C,,) results in loss of methionine acceptor activity. In this paper, we show that inactive tRNAfh”’ containing the C,, + U,, base change inhibits aminoac...

Nucleic acids containing double-headed nucleotides with additional nucleobases attached to the 59(S)position of thymidine through a methylene linker are studied. The additional bases are oriented towards base-base interactions in the minor groove of the DNA-double helix. Two new examples with adenine or cytosine as the additional bases as well as an analogue with a 4N-methylpiperazine in the sa...

Phosphoramidite reagents can phosphitylate guanine bases at the O6-position during solid phase synthesis and serious chain cleavage occurs if the base phosphitylation is not eliminated before the iodine/water oxidation step. This can be accomplished by blocking the O6-position with a 2-cyanoethyl protecting group for deoxyribonucleotides or with a p-nitrophenylethyl group for ribonucleotides, r...

The MukB protein is essential for chromosome partitioning in Escherichia coli and consists of 1484 amino acid residues (170 kDa). We have determined the base changes at the mutated sites of the mukB106 mutant and a newly isolated mutant, mukB33. These mutant mukB genes were each found to carry a single base-pair transition which leads to an amino acid substitution; a serine residue at position ...

Purpose: To study the simultaneous effects of prone position and anesthesia on intraocular pressure (IOP) and the time impact on post anesthesia visual loss development in percutaneous nephrolithotomy (PCNL). Materials and Methods: Twenty patients who were candidates for PCNL were recruited in this study. Intraocular pressure was measured in five occasions: 1. Base line; 2. Ten minutes after an...

abstract: this study uses esi indicators and statistics database for comparative evaluation of scientific production of iran, turkey, egypt and pakistan during 2002- 2012. this study is a survey and is done scientometrics approaches. spss software used for analysis of findings. toky and pearson correlation test was performed. study population was leader islamic countries who indexed base of esi...

Precise object manipulation by a robot requires precise knowledge of the position of the robot endeffector relative to the object. By the so-called eye-to-hand coordination, both the position of the object and the position of the robot relative to the camera are determined. In practice, usually the position of the robot base to camera is calibrated in advanced and the position of the robot ende...

The results of normal-mode analyses are in accord with the proposal that a low-frequency motion of the HhaI methyltransferase enzyme is responsible for base flipping in bound DNA. The vectors of the low-frequency normal mode of residues Ser-85 and Ile-86 point directly to the phosphate and ribose moieties of the DNA backbone near the target base in position to rotate the dihedral angles and fli...

Centralized network security management is the practice of funneling the vast amount of security-related data from the various sources in the network through a centralized process and personnel. This effort ensures a comprehensive view of the network security status. It promotes good communication and redundancy in analysis. Centralized network security management also provides the capability t...