The objective of this retrospective field study was to determine the effects of storing, for up to 62 h, heterospermic and homospermic semen in the short-term extender Beltsville thawing solution (BTS), on reproductive performance in Norwegian swine of four different breed combinations. The study was based on fertility records after single or double inseminations with semen collected at an AI s...

Background: Ram sperm have a higher polyunsaturated fatty acids and a lower cholesterol/phospholipid ratio than other species which renders the sperm much more vulnerable to oxidative damage caused by reactive oxygen species (ROS). Oxidative stress can affects the integrity of cells at both the structural and sub structural levels. Therefore, a suitable antioxidant may prevent cryo-injuries and...

this study aimed to investigate the effect of different concentration of zinc nano-complex on bull spermatozoa quality and pregnancy outcome after freeze-thawing process. ejaculates were collected from four holstein bulls, twice a week during four weeks. semen samples diluted with bioxcell extender containing zero (control), 10-6, 10-5, 10-4, 10-3 and 10-2 m of zinc nano-complex. the samples we...

Animal origin egg yolk extender has been associated with microbial contamination and interference with microscopic examination. Therefore, replacement of such extender with plant origin compound is inevitable without compromising the desired qualities of an ideal extender. In the current experiment, we compared the effect of soy-based extender (25% and 50%) of five adult Holstein-Friesian bulls...

The objective of the present study was to determine the effect of non-enzymatic antioxidants (vitamin C or E) in tris-citric acid buffer (TCA) on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa. Split pooled buffalo bull ejaculates were diluted in TCA egg yolk glycerol extender containing either vitamin C (TCAC), vitamin E (TCAE) or without antioxidant (TCAN) at 37°C. Extended s...

the aim of the current study was to evaluate the effects of the guanidinoacetic acid (gaa) supplement on the semen freezability of broiler breeder roosters. experiments were performed with twenty ross commercial strain broiler breeder roosters in four treatments and five repeats in a complete randomized design. roosters were fed a diet containing 0% (control), 0.06%, 0.12% and 0.18% gaa supplem...

objective: the aim of the present study was to evaluate three thawing rates on the post thaw motility, viability and chromatin structure of buffalo semen frozen in 0.5-ml straws. materials and methods: in this experimental study semen was collected with artificial vagina (42˚c) from four buffalo bulls.split pooled ejaculates (n=4) were extended at 37˚c with a bioxcell® extender. semen was coole...

The present study was conducted to observe the effect of increased osmolality of basic tris extender supplemented with trehalose and sucrose on post-thawing quality (motility, progressive motility, viability, the rate of acrosome abnormality, total abnormality and membrane integrity) of Markhoz goat spermatozoa. Fresh semen samples were evaluated for motility and sperm concentration. Only semen...

BACKGROUND The effects of seminal plasma on the presence of the cytokines transforming growth factor (TGF)-β1, interleukin (IL)-10 and IL-6 in ovarian follicles and follicular fluid were studied shortly after insemination in gilts.Ovaries from gilts were sampled 5-6 h after insemination with either seminal plasma (SP), fresh semen in extender (Beltsville thawing solution, BTS), spermatozoa in e...

egg yolk is the most commonly used cryoprotective agent for sperm cryopreservation of several species.the objective of this experiment was to compare the effectiveness of egg yolk from four avian species(domestic chicken, duck, turkey and pigeon) on sperm quality following cryopreservation of the ram semen.ejaculates were collected, using an electroejaculator, from six fertile rams. samples wer...