نتایج جستجو برای: bead array

تعداد نتایج: 140843  

Journal: :Journal of visualized experiments : JoVE 2014
Michelle R Emond James D Jontes

Cell-cell adhesion is fundamental to multicellular life and is mediated by a diverse array of cell surface proteins. However, the adhesive interactions for many of these proteins are poorly understood. Here we present a simple, rapid method for characterizing the adhesive properties of putative homophilic cell adhesion molecules. Cultured HEK293 cells are transfected with DNA plasmid encoding a...

2008
Eiichi Sekiyama Yumiko Matsuyama Daisuke Higo Takashi Nirasawa Masaya Ikegawa Shigeru Kinoshita Kei Tashiro

protein identification (Koo et al., 2005; Cheng et al., 2005; Mirr et al., 2005; Gruns et al., 2005; Tomosugi et al., 2005; Kijlstra et al., 1989; Zhou et al., 2006; Li et al., 2005; Fung et al., 2004; de Souza et al., 2006), but these protocols are sometimes hampered by the small amount of tear fluid and its low protein concentration. For high-throughput analysis, surface-enhanced laser desorp...

Journal: :Molecular & cellular proteomics : MCP 2010
Oliver Poetz Tanja Henzler Michael Hartmann Cornelia Kazmaier Markus F Templin Thomas Herget Thomas O Joos

Microarray-based sandwich immunoassays can simultaneously detect dozens of proteins. However, their use in quantifying large numbers of proteins is hampered by cross-reactivity and incompatibilities caused by the immunoassays themselves. Sequential multiplex analyte capturing addresses these problems by repeatedly probing the same sample with different sets of antibody-coated, magnetic suspensi...

Journal: :Biosensors & bioelectronics 2005
Shifeng Li Pierre N Floriano Nicolaos Christodoulides David Y Fozdar Dongbing Shao Mehnaaz F Ali Priya Dharshan Sanghamitra Mohanty Dean Neikirk John T McDevitt Shaochen Chen

This paper presents disposable protein analysis chips with single- or four-chamber-constructed from poly(dimethylsiloxane) (PDMS) and silicon. The chips are composed of a multilayer stack of PDMS layers that sandwich a silicon microchip. This inner silicon chip features an etched array of micro-cavities hosting polymeric beads. The sample is introduced into the fluid network through the top PDM...

2016
Bettina Schlick Petra Massoner Angelika Lueking Pornpimol Charoentong Mirjam Blattner Georg Schaefer Klaus Marquart Carmen Theek Peter Amersdorfer Dirk Zielinski Matthias Kirchner Zlatko Trajanoski Mark A. Rubin Stefan Müllner Peter Schulz-Knappe Helmut Klocker Aamir Ahmed

BACKGROUND Chronic inflammation is frequently observed on histological analysis of malignant and non-malignant prostate specimens. It is a suspected supporting factor for prostate diseases and their progression and a main cause of false positive PSA tests in cancer screening. We hypothesized that inflammation induces autoantibodies, which may be useful biomarkers. We aimed to identify and valid...

Journal: :Langmuir : the ACS journal of surfaces and colloids 2011
Peng Li Aamer Mahmood Gil U Lee

A new mode of transport is described that was capable of high-resolution separation of superparamagnetic materials from complex mixtures based on their size. Laminar flow and a rotating external magnetic field were applied to superparamagnetic beads assembled on a semiperiodic micromagnet array. Beads at the edge of the micromagnet array oscillated in-phase with the external magnetic field with...

2014
Burcu Ayoglu Eszter Szarka Krisztina Huber Anita Orosz Fruzsina Babos Anna Magyar Ferenc Hudecz Bernadette Rojkovich Tamás Gáti György Nagy Jochen M. Schwenk Gabriella Sármay József Prechl Peter Nilsson Krisztián Papp

The development of antigen arrays has provided researchers with great tools to identify reactivities against self or foreign antigens from body fluids. Yet, these approaches mostly do not address antibody isotypes and their effector functions even though these are key points for a more detailed understanding of disease processes. Here, we present a bead array-based assay for a multiplexed deter...

Journal: :The journal of physical chemistry letters 2017
Seulgi So Han Wai Millie Fung Kellen Kartub Adam M Maley Robert M Corn

Ordered nanocone arrays of the electroactive polymer poly(3,4-ethylenedioxythiophene) (PEDOT) were fabricated by the simultaneous oxygen plasma etching of an electrodeposited PEDOT thin film coated with a hexagonally closed packed polystyrene bead monolayer. PEDOT nanocone arrays with an intercone spacing of 200 nm and an average nanocone height of 350 nm exhibited a low broadband reflectivity ...

Journal: :Colloid and Polymer Science 2009
John Texter

Templating processes for creating polymerized hydrogels are reviewed. The use of contact photonic crystals and of non-contact colloidal crystalline arrays as templates are described and applications to chemical sensing and device fabrication are illustrated. Emulsion templating is illustrated in the formation of microporous membranes, and templating on reverse emulsions and double emulsions is ...

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