Cell-based biosensors (CBBs) are becoming important tools for biosecurity applications and rapid diagnostics in food microbiology for their unique capability of detecting hazardous materials. Pollutants, such as heavy metals and chemicals, are now considered as a global threat and are associated with detrimental health outcomes. Fast and accurate detection of pollutants is essential to reduce t...

Bioluminescence (BL) relies on the enzymatic reaction between luciferase, a substrate conventionally named luciferin, and various cofactors. BL imaging has become widely used technique to interrogate gene expression cell fate, both in small large animal models of research. Recent developments include generation improved luciferase–luciferin systems for deeper more sensitive as well new caged lu...

objective(s): sirna may be a very promising tool for treatment of various diseases especially in cancer therapy due to high specificity. one of the main hurdles applications of sirnas in vivo is optimization of the delivery strategy, especially the carrier systems. the aim of this study was to optimize sirna delivery into suspended erythroleukemic cell line k562. materials and methods: we appli...

To aid in the interpretation of high-throughput screening (HTS) results derived from luciferase-based assays, we used quantitative HTS, an approach that defines the concentration-response behavior of each library sample, to profile the ATP-dependent luciferase from Photinus pyralis against more than 70,000 samples. We found that approximately 3% of the library was active, containing only compou...

The possibility of using firefly luciferase as a substrate for an aspartic proteinase was explored. Several amino acid modifications to the C-terminus of the luciferase were created on the basis of the known substrate of the Arabidopsis thaliana aspartic proteinase, pro-(barley lectin). One luciferase with the sequence Arg-Asp-Gly-Val-Phe-Ala-Ala instead of the native Arg-Glu-Ile-Leu-Ile-Lys-Al...

BACKGROUND The Gaussia princeps luciferase is used as a stand-alone reporter of transgene expression for in vitro and in vivo expression systems due to the rapid and easy monitoring of luciferase activity. We sought to simultaneously quantitate production of other recombinant proteins by transcriptionally linking the Gaussia princeps luciferase gene to other genes of interest through the foot-a...