Establishment of efficient protocol for the induction of somatic embryogenesis and plant regeneration from embryonic axes explants was achieved in Glycine max (L.) Merr. Highest frequency (92.9%) of embryogenic callus formation was obtained from embryonic axes explants of cv. CO-1 on MS media containing 2,4-Dichlorophenoxy acetic acid (2,4-D) and 6-benzyl adenine (BA). Fast growing, yellowish n...

the complexes of mn(oac)3 and/or mn(acac)3 with nucleic bases and nucleosides (adenine, guanine, xanthine, adenosine and guanosine) have been synthesized in nonaqueous solution. polarographic and spectroscopic (ir and visible) methods have been used to establish the active site(s) on the imidazole and pyrimidine rings in the nucleic bases and nucleosides for the interaction with the mn(iii) com...

The electrochemical behavior of 6-benzyl adenine (6-BA) has been studied in 0.1 mol L(-1) HAc-NaAc solution (pH 3.8). Cyclic voltammetry, single-sweep polarography and direct current polarography were employed to clarify the mechanism of the electrode process; the kinetic parameters of the rate-determining step were determined. Reduction of 6-BA involves two pH-dependent processes, correspondin...

CO oxidoreductase was purified to 95% homogeneity from crude mycelial extracts of Streptomyces G26. The purified preparation has a specific activity of 25.7 units/mg, a 13-fold improvement on crude soluble mycelial extracts. The native enzyme (Mr 282,000) is composed of non-identical subunits of Mr 110,000 and 33,000. It is a molybdenum hydroxylase containing 1.6 mol of FAD, 7.3 mol of Fe, 8.3 ...

Background: Okra [Abelmoschus esculentus (L.) Moench.] is a nutrient-rich vegetable crop widely grown in the tropics and sub-tropics mainly for its edible pods. The haploid technique has been used plant breeding improvements of plants to develop new varieties relatively short time. Hence, we have optimized several factors such as growth regulators (PGR), sucrose concentration, cold treatment, t...

Micropropagation for extensive production of elite planting materials is an effective and superior alternative to suckers conventional means propagation Musa spp. The present investigation was carried out evaluate the genetic identity different micropropagated banana genotypes Grand Naine, Shrimantee Basrai. Further Naine comparing with mother plant during stages subculture using Simple Sequenc...

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Pollen extracts of date palm were used in tissue culture medium for banana as growth substances in comparison with growth regulators. Pollen extracts contain auxin (IBA) and tryptophan (auxin precursor). Water pollen extract (WPE) contains higher concentration of indoles compared with ethanol pollen extract (EPE). Most of treated plants showed highly growth characteristics such as shoot number,...

The present investigation was carried out to evaluate the level of salt toxicity on four novel chickpea genotypes (BG 372, BG 1105, BG 2019 and BG 2024) under in vitro condition for percentage seed germination and morphogenesis. All the four genotypes were exposed to different concentrations (0, 1, 50, 100 and 150 mM) of NaCl in the Murashige & Skoog (MS) medium supplemented with or without ben...

Clostridium pasteurianum cell-free extracts enzymatically reduced metronidazole when coupled by hydrogenase via reduced ferredoxin. A 5 mM concentration of methyl viologen, flavin adenine dinucleotide, or flavin mononucleotide could completely replace ferredoxin (0.05 mM) in the in vitro reduction assay system, whereas 5 mM benzyl viologen was less effective. However, when these electron carrie...