Intact BHK (baby hamster kidney) cells catalyze the hydrolysis of UDP-galactose to free galactose. The generation of galactose from UDP-galactose and its intracellular utilization impede the detection of possible galactosyl transferases on the cell surface of intact cells. Several independent procedures have been used to distinguish between intracellular and cell surface glycosyl transferases. ...

Carnosine is known as a natural dipeptide, which inhibits the proliferation of tumor cells throughout its action on mitochondrial respiration and cell glycolysis. However, not much about effects metabolism healthy cells. We explored Karnozin EXTRA® capsule with different concentrations L-carnosine, viability expressions intermediate filament vimentin (VIM) superoxide dismutase (SOD2) in normal...

Human glial cell line-derived neurotrophic factor (hGDNF) is the most potent dopaminergic described so far, and it therefore considered a promising drug for Parkinson's disease (PD) treatment. However, production of therapeutic proteins with high degree purity specific glycosylation pattern major challenge that hinders its commercialization. Although variety systems can be used protein producti...

Bivalent cations, such as CaZ+, Mgz+ or Mnz+, are required for the adhesion of cells t o serum-coated substratum, and MnZ+ appears t o be particularly effective in this respect (Rabinowitch & DeStefano, 1973; Yasuda, 1974). We report here the stimulation by bivalent cations of adhesion of BHK-21/CI 3 cells (BHK) and their polyoma-transformed derivatives (PyBHK) to serum-coated glass by using an...

In this study, the immunogenicity of a rabies vaccine that was used in one of the Isfahan cattle farms is reported. This vaccine was produced by replication of the Pasteur virus (PV) strain on BHK-21 monolayer cell culture. The virus suspension was inactivated by ß-propiolactone treatment and the harvested pool showed sterility and safety. Thus, the obtained vaccine was tested on mice and a sat...

The sensitivities of BHK-21 (C-13) and murine neuroblastoma (C-1300; clone NA) cells for the isolation of small quantities of a street strain rabies virus were compared. Suspensions of brain from mice sacrificed prior to the onset of clinical signs of rabies were used to stimulate weakly positive diagnostic specimens. The results of cell culture isolation were compared with those of the direct ...

In BHK cells infected with pseudorabies virus, there was a substantial increase in the phosphorylation of ribosomal protein S6. This increase occurred between 2 and 4 h after infection and persisted at least until 9 h. We estimated that in mock-infected cells S6 contained, on an average, one phosphate group per protein chain, whereas in infected cells this rose to between four and five phosphat...

The RNA polymerase activity present in the cytoplasm of BHK cells infected with vaccinia virus is not affected by rifampicin or by alpha-amanitin.

the aim pf the present study was to compare three different methods for detection of food and mouth disease virus antigens(a, asia1, o1)from mouth epithelium samples and also to monitore the distribution of fmdv serotypes 338 cattle and 34 sheep of iran during 2000-2001. the suspension fluids of processed tongue epithelium samples were clarified by centrifugation and tested for presence of fmdv...

Microtubule breakdown in the presence of 5 or 40 microg/ml of colchicine is observed in BHK-21/C13 fibroblast-like cells. Several morphological and physiological effects are noted in the absence of microtubules: (a) the cells transform from fibroblast-like to epithelial-like cells; (b) the normal pattern of intracellular birefringence changes and a juxtanuclear cap of birefringent filaments is ...