Methods enabling the delivery of proteins into eukaryotic cells are essential to address protein functions. Here we propose broad applications to cell biology for a protein delivery tool based on bacterial type III secretion (T3S). We show that bacterial, viral, and human proteins, fused to the N-terminal fragment of the Yersinia enterocolitica T3S substrate YopE, are effectively delivered into...

We report the solution structure of BID, an intracellular cross-talk agent that can amplify FAS/TNF apoptotic signal through the mitochondria death pathway after Caspase 8 cleavage. BID contains eight alpha helices where two central hydrophobic helices are surrounded by six amphipathic ones. The fold resembles poreforming bacterial toxins and shows similarity to BCL-XL although sequence homolog...

Title of dissertation: DESIGN OF DISCRETE AUCTION Pacharasut Sujarittanonta, Doctor of Philosophy, 2010 Dissertation directed by: Professor Peter Cramton Department of Economics Chapter 1: Efficient Design of an Auction with Discrete Bid Levels This paper studies one of auction design issues: the choice of bid levels. Full efficiency is generally unachievable with a discrete auction. Since ther...

Westudy independent private-value all-pay auctionswith risk-averse players. We show that: (1) Players with low values bid lower and players with high values bid higher than they would bid in the risk neutral case. (2) Players with low values bid lower and players with high values bid higher than they would bid in a first-price auction. (3) Players’ expected utilities in an all-pay auction are l...

B-vitamin assays with chicks. 2 89 Roberts, E. C. & Snell, E. E. (1946). J. bid. Chem. 163, 499. Rosen, F., Huff, J. W. & Perlzweig, W. A. (1946). J. bid. Chem. 163, 343. Snell, E. E. & Rannefeld, A. N. (1945). J. bid. Chem. 157, 475. Sreenivasan, A., Harper, A. E. & Elvehjem, C. A. (1949). J. bid. Chem. 177, 117. Stokstad, E. L. R., Jukes, T. H., Pierce, J. . Page, A. C. & Franklin, A. L. (194...

I n the classic revenue management (RM) problem of selling a fixed quantity of perishable inventories to price-sensitive non-strategic consumers over a finite horizon, the optimal pricing decision at any time depends on two important factors: consumer valuation and bid price. The former is determined exogenously by the demand side, while the latter is determined jointly by the inventory level o...

Sealed-Bid auction is an efficient and rational method to establish the price in open market. However sealed-bid auctions are subject to bid-rigging attack. Receipt-free mechanisms were proposed to prevent bid-rigging. The prior receipt-free mechanisms are based on two assumptions; firstly, existence of untappable channel between bidders and auction authorities. Secondly, mechanisms assume the ...

Overexpression of the BH3-interacting domain death agonist (BID) protein sensitizes certain cancer cell lines to apoptosis induced by anticancer agents, particularly by those acting through death receptors (e.g. TRAIL). Previously, we showed that recombinant BID fused with TAT cell penetrating peptide (TAT-BID) allowed for controlled delivery of BID to different cancer cell lines and moderately...

Consider a retailer using sponsored search marketing together with dynamic pricing. The retailer’s bid on a search keyword affects the retailer’s rank among the search results. The higher the rank, the higher the customer traffic and the customers’ willingness-to-pay will be. Thus, the question arises: When a retailer bids higher to attract more customers, should the accompanying price also dec...