The ability of various nonionic detergents to solubilize D-alanine carboxypeptidase and other membrane-bound enzymes was correlated to a physical property of the detergent, the HLB number. Only a fairly narrow range of detergents were effective solubilizing agents. Purified carboxypeptidase required either a detergent or detergent plus a lipid fraction for stability. Only those detergents effec...

Previously, toxicity of some synthetic detergents (including laundry detergents) to the plant seedlings several species terrestrial higher plants was discovered in research conducted at Moscow University by S.A.Ostroumov. A new example a detergent found this study. The tested, namely liquid (LLD) “Blue Moon”, which manufactured Blue Moon Group Co, Ltd (Guangzhou, China), produced noticeable phy...

Introduction – Eutrophication is a serious problem This article compares the toxic effects of detergents of different types on aquatic organisms and is intended to outline the research comparing the toxicity of phosphate detergents with the toxicity of zeolite ones. Particular attention is given to the comparison of zeolitic and phosphate detergents. Phosphates are an important component in syn...

Microbial lipases are an important group of biotechnologically valuable enzymes, because of the versatility of their applied properties and ease of mass production. Lipases of microbial origin are widely diversified in their enzymatic properties and substrate specificity, which make them very attractive for industrial applications. This review describes the applications of microbial lipases in ...

There is a limited number of reports regarding detergents and proteases inactivating, degrading, or destabilizing abnormal prion protein (PrPSc). In the present study, the effect of alkaline detergents and proteases on the breakdown of PrPSc in the absence of proteinase K (PK) (degradation) and the presence of PK (destabilization) was investigated. PrPSc from brain homogenate of terminally-dise...

Commercial fungal amylase (diastase) has been immobilized through diazotization onto zirconia coated arylamine glass beads, affixed inside a plastic beaker. The immobilized enzyme retained 73.3% of the initial activity of free enzyme with a conjugation yield of 8 mg/g. Optimum pH of enzyme was decreased, while temperatures for maximum activity, energy of activation, time of incubation and Km fo...

Spiralin could not be solubilized in the absence of detergents, and it was shown by charge-shift crossed immunoelectrophoresis that this protein was capable of binding detergents under nondenaturing conditions. These properties indicate the amphiphilic nature of spiralin, which therefore should be regarded as an intrinsic membrane protein. The efficiency of mild (ionic and neutral) detergents t...

Specific degradation of the phospholipid membrane of guinea-pig liver microsomal fraction with phospholipase A inactivated glucuronyltransferase. The inactivation was reversed by phosphatidylcholine and mixed microsomal phospholipid micelles at concentrations similar to those present in intact microsomal preparations. The other commonly occurring phospholipids did not reactivate phospholipase A...

1. The members of the homologous series of anionic detergents, the sodium salts of the sulfated straight chain alcohols with the general formula C(n)H(2n+1).SO(3).Na, are hemolytic, the lytic activity being at a maximum when the compound contains 14 carbon atoms in the chain. In systems in which lysis is comparatively rapid, the hemolytic effect increases with increasing pH, but in systems cont...

OBJECTIVE To analyse paediatric exposures to pod and traditional laundry detergents in Italy and changes in exposure trends. METHODS Analyses of a series of patients aged <5 years and exposed to laundry detergents between September 2010 and June 2015, identified by the National Poison Control in Milan. RESULTS In comparison with patients exposed to traditional laundry detergents (n=1150), a...