A cholinesterase based biosensor was constructed in order to assess the effects of ionizing radiation on exposed AChE. Although the primary objective of the experiment was to investigate the effect of ionizing radiation on the activity of the biosensor, no changes in cholinesterase activity were observed. Current provided by oxidation of thiocholine previously created from acetylthiocholine by ...

Lactate detection plays a significant role in healthcare, food industries and is specially necessitated in conditions like hemorrhage, respiratory failure, hepatic disease, sepsis and tissue hypoxia. Conventional methods for lactate determination are not accurate and fast so this accelerated the need of sensitive biosensors for high-throughput screening of lactate in different samples. This rev...

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This paper describes the application of electrochemical disposable biosensors in food analysis, which have recently been developed in our laboratory. Disposable biosensors, based on acetylcholinesterase inhibition activity, were exploited for testing the presence of organophosphorus and carbamate pesticides in water, fruit, and vegetable samples. The paper further describes preliminary tests fo...

Biosensors are analytical devices being approachable for multiple analytes assay. Here, biosensors with intercepted acetylcholinesterase (AChE) or butyrylcholinesterase (BChE) are presented as tool for assay of anticholinergic compounds such as pesticides, nerve agents and some natural toxins. Principle of assay is based on evaluation of cholinesterase activity and its pertinent decrease in pre...

Biosensors can be excellent analytical tools for monitoring programs working to implement legislation. In this article, biosensors for environmental analysis and monitoring are extensively reviewed. Examples of biosensors for the most important families of environmental pollutants, including some commercial devices, are presented. Finally, future trends in biosensor development are discussed. I...

We further examined the usefulness of previously reported Bacillus subtilis biosensors for antibacterial mode-of-action studies. The biosensors could not detect the tRNA synthetase inhibitors mupirocin, indolmycin, and borrelidin, some inhibitors of peptidoglycan synthesis, and most membrane-damaging agents. However, the biosensors confirmed the modes of action of several RNA polymerase inhibit...

Recent progress in third-generation electrochemical biosensors based on the direct electron transfer of proteins is reviewed. The development of three generations of electrochemical biosensors is also simply addressed. Special attention is paid to protein-film voltammetry, which is a powerful way to obtain the direct electron transfer of proteins. Research activities on various kinds of biosens...

Heavy metals are among the most serious pollutants, and thus there is a need to develop sensitive and rapid biomonitoring methods for heavy metals in the environment. Critical parameters such as bioavailability, toxicity and genotoxicity cannot be tested using chemical analysis, but only can be assayed using living cells. A whole-cell biosensor uses the whole cell as a single reporter incorpora...

Nanotechnology is currently playing a significant role in the development of biosensors. The sensitivity and performance of biosensors improves drastically by incorporating nanomaterials and adopting new signal transduction technologies. With the rapid development of tools and fabrication processes , nanoscale sensors capable of interacting with extremely small molecules have been developed. Th...