Monocytes play an important role in the immune system and are responsible for phagocytizing and degrading foreign microorganisms in the body. The isolation of monocytes is important in various immunological applications such as in-vitro culture of dendritic cells. We present a magnetophoretic-based microfluidic chip for rapid isolation of highly purified, untouched monocytes from human blood by...

We develop the Kernel Multitask Latent Analysis (KMLA) method for modeling many-to-many relationships between inputs and responses, and show how it can be applied to inductive transfer problems in bioseparations. KMLA performs dimensionality reduction targeted towards a multitask loss function much like Kernel Partial Least Squares (KPLS). KPLS is limited to least squares multiple regression wh...

BACKGROUND Fe₃O₄-gold-chitosan core-shell nanostructure can be used in biotechnological and biomedical applications such as magnetic bioseparation, water and wastewater treatment, biodetection and bioimaging, drug delivery, and cancer treatment. RESULTS Magnetite nanoparticles with an average size of 9.8 nm in diameter were synthesized using the chemical co-precipitation method. A gold-coated...

The effect of ionic strength of agarose solution and quenching temperature of the emulsion on the structure and mechanical strength of agarose-based chromatographic adsorbents was investigated. Solutions of agarose containing different amounts of NaCl were emulsified at elevated temperature in mineral oil using a high-shear mixer. The hot emulsion was quenched at different temperatures leading ...

Macroor micro-porous chitosan membranes have been utilized as adsorptive membrane for the removal of metal ions, organic macromolecules or bioseparation of protein mixtures. The removal or separation mechanism is not based on physical size retention but based on the high adsorption capability of chitosan to the target solutes. For this purpose, it is important that large macro or micro pores an...

Since magnetic particles have unique features, the development of a variety of medical applications has been possible. The most unique feature of magnetic particles is their reaction to a magnetic force, and this feature has been utilized in applications such as drug targeting and bioseparation including cell sorting. Recently, magnetic nanoparticles have attracted attention because of their po...

Encapsulation techniques are fmding increasing interest in a variety of areas of biotechnology. Enzymes, living ceils, hormones, drugs, adsorbents and other bioactive materials can be encapsulated and used in bioreactors, artificial organs, controlled drug-release systems, bioseparation systems and etc (Chang, 1972; Kondo, 1979; Lira, 1984; Nigam, 1988; Nixon, 1976). Ideally, encapsulation of b...

Herein we present a gravity-driven microdevice furnished with tunable TiO2 nanotube arrays (TNAs) inside as the separation medium for consecutive on-chip enrichment and isotope labeling of serum phosphopeptides. The 3D tubular architectures of TNAs dramatically enhanced the affinity towards phosphate-containing molecules and also provided a spacious microenvironment for isotope dimethyl labelin...

Nanotechnology is a fast-growing area, involving the fabrication and use of nano-sized materials and devices. Various nanocomposite materials play a number of important roles in modern science and technology. Magnetic and fluorescent inorganic nanoparticles are of particular importance due to their broad range of potential applications. It is expected that the combination of magnetic and fluore...

Superamphiphobic nanofibrous membranes exhibiting robust water/oil proof and breathable performances were prepared by the combination of a novel synthesized fluorinated polyurethane (FPU) containing a terminal perfluoroalkane segment and incorporated SiO(2) nanoparticles (SiO(2) NPs). By employing the FPU/SiO(2) NPs incorporation, the hybrid membranes possess superhydrophobicity with a water co...