Blackleg disease, caused by the fungal pathogen Leptosphaeria maculans, is most devastating disease of canola (Brassica napus, oilseed rape) worldwide. Breeding for genetic resistance widely used tool controlling this and minimizing impact on yield. To date, five genes (Rlm2, LepR3, Rlm4, Rlm7, Rlm9) have been cloned from B. representing alleles two different gene loci, Rlm2-LepR3 Rlm4-7-9. We ...

background: recent studies indicate an increased incidence of pertussis disease in recent years. the aim of this study was to evaluate the efficacy of the acellular vaccine for children (as a replacement of current whole cell vaccine in the expanded program on immunization) and for high-risk adults in iran through updating current best available evidence.   methods: we performed a systematic li...

Improved conventional PCR techniques are required for the rapid on-site detection of human and animal diseases. In this context, a PCR method using dry-stored reagents intended for the detection of Clostridium spp. is presented. Basic PCR reagents (BSA, PCR buffer, MgCl₂ and primers), which were dried on polyolefin matrices, showed stability at ambient temperatures for up to 10 months without a...

The distribution of pathogenic anaerobes in the environment and the relationship with diseases in animals are discussed. A distinction between the spore-bearing anaerobes (clostridia) and the Gram-negative non-spore-forming anaerobes is necessary. The main habitat of clostridia is the soil but they are also found in dust, sewage, rivers, lakes, sea water, milk, vegetables, fresh meat, fish, ins...

background as per the vaccine management policy of the government of india all vaccine vials opened for an immunization session were discarded at the end of that session, irrespective of the type of vaccine or the number of doses remaining in the vial prior to 2013. subsequently, open vial policy (ovp) was introduced in 2013 and should reduce both vaccine wastage as well as governmental healthc...