Tissue plasminogen activator is an endogenous fibrin-specific serine protease with potent thrombolytic activity. We investigated the efficacy of tissue plasminogen activator in reducing cerebral infarct size after thromboembolic stroke in a rabbit model. Seventeen rabbits were randomized to receive either tissue plasminogen activator (2.5 mg/kg, n = 6) or vehicle control (n = 11). We controlled...

Abstract Factor D (FD) is an essential element of the alternative pathway complement system, and it circulates predominantly in cleaved, activated form blood. In resting blood, mannose-binding lectin–associated serine protease 3 (MASP-3) exclusive activator pro-FD. Similarly to FD, MASP-3 also mainly active form. It was not clear, however, how zymogen activated. To decipher its activation mecha...

Peripheral blood cell preparation from 23 normal subjects and 72 patients with acute and 32 patients with chronic myeloid leukemia were cultured in vitro and released plasminogen activators were analyzed. The quantity of plasminogen activator secreted by leukemic cells varied widely and could not be correlated with the clinical severity of the disease. Immunochemical and electrophoretic techniq...

tenecteplase is a variant of tissue plasminogen activator (t-pa) which has better pharmacokinetic properties and more selective thrombolytic activity. in the present study, we describe a rapid method to introduce three sets of mutation into defined positions in t-pa cdna by a site-directed mutagenesis based on a megaprimer pcr approach to produce tenecteplase coding sequence where amino acids a...

standard protocols should be established for treating eligible stroke patients with tissue plasminogen activator (tpa) (recommendation class i, level of evidence b). the iranian standard protocol of intravenous thrombolysis with recombinant tissue plasminogen activator (ivttpa) is the best possible and easy to use method for performing intravenous thrombolysis in iran. this protocol overcomes p...

the aim of the present research was to study the effects of regular training and exhaustive exercise on t-pa/pai-1 complex in the blood of forty eight rats. they were divided randomly into control and experimental groups and the subgroups included the primary acute, secondary acute and 8-wk regularly trained (each group 8 rats). endurance training programs (five days a week, speed 15-22 m/min f...

BACKGROUND We recently assigned a new fibrinolytic function to cell-derived microparticles in vitro. In this study we explored the relevance of this novel property of microparticles to the in vivo situation. DESIGN AND METHODS Circulating microparticles were isolated from the plasma of patients with thrombotic thrombocytopenic purpura or cardiovascular disease and from healthy subjects. Micro...

Arterial occlusion of the upper limb did not affect the fibrinolytic activity in the venous blood of that limb; venous occlusion associated with venous distension resulted in a marked rise in activity. Local fibrinolytic activity was increased substantially by active exercise; passive exercise induced a smaller increase. Active exercise undertaken during arterial occlusion did not result in a r...

OBJECTIVE(S) Snake venoms contain complex mixture of proteins with biological activities. Some of these proteins affect blood coagulation and platelet function in different ways. Snake venom toxin may serve as a starting material for drug design to combat several pathophysiological problems such as cardiovascular disorders. In the present study, purification of anticoagulation factor from venom...

the regulation of fibrinolysis in the blood vessel by producing plasminogen activators and plasminogen activator inhibitors. Defects in physiological regulation of the balance of the activities between plasminogen activator and the inhibitors may cause vascular diseases such as thromboembolismand atherosclerosis. In patients with such diseases, the inhibitor may be dominant in this balance. Act...