Blood-Contacting Surfaces In article number 2102214, Jun Ki Hong, Anna Waterhouse, Chiara Neto, and co-workers report that tethered-liquid perfluorocarbons show low adhesion of blood components. The cover image is a scanning electron micrograph revealing minimal platelets after incubation in human whole blood. bumpy background the tethered-perfluorocarbon layer which stabilizes liquid perfluoro...

RATIONALE Several mutations that impair the development of blood lineages in the mouse also impair the formation of the lymphatic vasculature and its separation from the blood vasculature. However, the basis for these defects has remained unknown because the mutations characterized affect more than one blood lineage. OBJECTIVE We tested the hypothesis that megakaryocytes/platelets are require...

Abstract Platelets, as nonnucleated blood components, are classically recognized for their pivotal role in hemostasis. In recent years, however, accumulating evidence points to a nonhemostatic platelets, active participants the inflammatory and immune responses microbial organisms infectious diseases. This stems from ability of activated platelets secrete plethora immunomodulatory cytokines che...

Rationale: Several mutations that impair the development of blood lineages in the mouse also impair the formation of the lymphatic vasculature and its separation from the blood vasculature. However, the basis for these defects has remained unknown because the mutations characterized affect more than one blood lineage. Objective: We tested the hypothesis that megakaryocytes/platelets are require...

Platelets may become activated in a number of clinical disorders and participate in thrombus formation. We developed a direct test for activated platelets in whole blood using flow cytometry. Whole blood was incubated with either biotin-PAC1, a monoclonal antibody specific for the fibrinogen receptor on activated platelets, or biotin-S12, an antibody specific for an alpha-granule membrane prote...

Platelets will normally adhere to ‘damaged’ vessel walls and to other ‘activated’ platelets in flowing blood (1); platelets adhere from flowing whole blood onto vessel segments (2) and onto solid surfaces in flow chambers coated with extracellular matrix or purified adhesive proteins ( 3-5). Neutrophils can adhere to each other and to platelets in flowing suspension (6) or adhere to platelets i...