Detection of animals which are persistently-infected with bovine virus diarrhoea virus (BVDV) is of prime importance in the control of pestivirus infections in cattle, as these animals constitute the main reservoir of the virus. Identification of such animals can be readily performed using crude whole blood samples with a sandwich enzyme-linked immunosorbent assay (ELISA) requiring only approxi...

conclusions the newly developed simple indirect elisa showed high sensitivity and specificity with respect to vnt. developing such a simple, sensitive, and specific elisa which is much less expensive than the available commercial elisa kits can improve the detection of bvdv infections, help to eliminate the disease from herds, and decrease economic losses caused by this disease. background bovi...