We developed Diversity Array Technology (DArT) markers for application in genetic studies of Brassica napus and other Brassica species with A or C genomes. Genomic representation from 107 diverse genotypes of B. napus L. var. oleifera (rapeseed, AACC genomes) and B. rapa (AA genome) was used to develop a DArT array comprising 11 520 clones generated using PstI/BanII and PstI/BstN1 complexity re...

The number of major rDNA loci (the genes coding for 18S-5.8S-26S rRNA) was investigated in the economically important Brassica species and their wild relatives by in situ hybridization of an rDNA probe to metaphase chromosomes and interphase nuclei. The diploid species B. nigra (B genome) has two major pairs of rDNA loci, B. oleracea (C genome) has two major pairs and one minor pair of loci, wh...

Canola is an offspring of rapeseed (Brassica napus and Brassica campestris/rapa), which was bred through standard plant breeding techniques to have low levels of erucic acid and glucosinolates. Canola seed is rich in oil, and after oil extraction, the remaining “canola meal” (CM), is a rich protein source used as feedstock to different animal species, mainly dairy cows in North America and in E...

Various effects on the separation of raw reaction mixture after the ethanolysis of rapeseed oil were investigated. Water addition, initial temperature of separation, centrifugation, ionic compound addition and time effects affecting concentrations of potassium, free glycerol and yield of the ester phase were found out. Centrifugation has the most positive effect. Finally, correlation between co...

Glucosinolates are sulphur containing secondary metabolites biosynthesized by many plant species in the order Brassicales. Physical tissue or cell injury leads to the breakdown of glucosinolates through the hydrolytic action of the enzyme myrosinase, resulting in the production of compounds including isothiocynates, thiocyanates and nitriles. Derivative compounds of glucosinolates have a wide r...