Patients with high-risk neuroblastoma (NB) initially respond to aggressive, alkylator-based therapy only to die from recurrent disease that is refractory to chemotherapy, including alkylating agents. We examined the ability of buthionine sulfoximine (BSO)-mediated glutathione (GSH) depletion to modulate melphalan (LPAM) resistance in five NB cell lines established after progressive disease foll...

Intracellular glutathione (GSH) has been implicated as a regulatory determinant of multidrug resistance protein (MRP) function. The objective of the present study was to evaluate in vivo the ability of d,l-buthionine-(S,R)-sulfoximine (d,l-BSO), a potent inhibitor of GSH biosynthesis, to reverse MRP-mediated drug resistance to doxorubicin. Athymic nude mice (nu/nu) bearing advanced parental hum...

We performed experiments on male Australian painted dragon lizards (Ctenophorus pictus) to test the hypothesis that carotenoids can scavenge reactive oxygen species (ROS), protecting the organism from oxidative stress, and that this capacity is reflected in skin colours involved in signalling. Subsequent to 4 weeks of carotenoid treatment we used flow cytometry to analyse unspecified ROS (H(2)O...

The full matrix of electro-elastic constants of sillenite-type crystals Bi12TiO20 (BTO) and Bi12SiO20 (BSO) were determined by the resonance method, with d14 and k14 being on the order of 40–48 pC/N and 31%–36%, respectively. In addition, double-rotated orientation dependence of d33 was investigated, with the maximum values of 25–28 pC/N being achieved in ZXtl45°/54°-cut samples. The electrical...

OBJECTIVE Diabetes elevates cardiovascular disease (CVD) risk more markedly in women than in men. Because the high risk of CVD among women with type 2 diabetes (DM2) may be partly due to increased ovarian androgen production, we investigated whether a history of bilateral salpingo oophorectomy (BSO) is inversely associated with CVD mortality among women with DM2. RESEARCH DESIGN AND METHODS D...

There exists a strong link between oxidative stress, renal dopaminergic system, and hypertension. It is reported that reactive oxygen species attenuate renal proximal tubular dopamine receptor (D1R) function, which disrupts sodium regulation and leads to hypertension. However, the mechanisms for renal D1R dysfunction are not clear. We investigated the role of redox-sensitive transcription facto...

Background: The high saturated fatty acid content of beef has been linked to obesity and cardiovascular diseases. study investigated the impact black seed oil (BSO) on fat-altered hemato-biochemical pathophysiological alterations in albino mice. Methods: Twenty four Swiss Albino mice (Mus musculus) 25-28 days old were equally divided into three groups (n=8) namely A, B C. Mice group A was desig...

Accumulated evidence over the past 10 years has strongly indicated that the fallopian tube is the site of origin for many high-grade serous carcinomas (1), which represent 50% to 60% of all epithelial ovarian cancers. This multi-origin model of ovarian cancer development has important implications for prevention of ovarian cancer: if some or all high-grade serous tumors develop in the fallopian...

With the expectation that trypanosomal glutathione (GSH) plays a major protective role against the endogenous oxidant stress that results form high intracellular levels of H2O2, we sought to deplete Trypanosoma brucei brucei of their GSH through inhibition of its biosynthesis. Administration of buthionine sulfoximine (BSO), a reversible inhibitor of gamma-glutamylcysteine synthetase, to parasit...

Propyl gallate (PG) as a synthetic antioxidant is widely used in processed food and medicinal preparations. It also exerts a variety of effects on tissue and cell functions. In the present study, we investigated the effects of L-buthionine sulfoximine (BSO, an inhibitor of GSH synthesis), diethyldithiocarbamate (DDC, an inhibitor of Cu/Zn-SOD) or 3-amino-1,2,4-triazole (AT, an inhibitor of cata...