Asparagus breslerianus a wild species in Iran, exhibited tolerance to salt in dry gypsum hills and dry lands. In order to check for salt tolerance thresholdvia in vitro conditions, the A. breslerianus callus was subjected to NaCl (sodium chloride) treatments. Six weeks old calli derived from male spear bud, were exposed to 0, 21.88, 43.76, 65.64, 76.58, 87.52, 109.40, 131.28, 153.16 and 175.04 ...

Unavailability of performant planting material pineapple constitutes a major problem its cultivation in Africa. For this purpose, indirect organogenesis technique is used to evaluate the vitro responses two cultivars during explant’s regeneration. Calli were induced from crown leaf and plantlets “Smooth Cayenne” “Sugarloaf cultivars”. Murashige Skoog medium with vitamins B5 supplemented differe...

A simple micropropagation protocol has been achieved using nodal region and apical leaf of Clerodendrum indicum,(L) kuntze; an important medicinal plant of Verbenaceae. The explants were cultured in MS medium supplemented with NAA, BA, 2,4-D,Kn, IBA in various combination and concentrations (0.110.0 μM). Response to callogenesis were diverse, ranging from white vigorous compact callus (1.0μM NA...

Preharvest sprouting of wheat (Triticum aestivum L.) is associated with inadequate seed dormancy. Although abscisic acid (ABA) has often been suggested to play a central role in developing seed, its involvement in dormancy of mature seed lacks firm experimental evidence and endogenous ABA levels are not well correlated with germinability. We examined genotypic and temporal variation in wheat se...

Although tremendous progress has been made in recent years in identifying molecular mechanisms of small interfering RNA (siRNA) functions in higher plants, the possibility of direct interaction between genomic DNA and siRNA remains an enigma. Such an interaction was proposed in the 'RNA cache' hypothesis, in which a mutant allele is restored based on template-directed gene conversion. To test t...

An efficient system was established for high frequency embryogenesis and regeneration of indica rice, Oryza sativa L. cv. MDU 5. Basic media, carbohydrate sources and concentrations, agar concentrations, amino acids, cytokinins and auxins were evaluated in callus induction and regeneration media to establish the most efficient regeneration protocol for MDU 5 indica rice. The optimized callus in...

The present study establishes a regeneration protocol and optimizes conditions for Agrobacterium-mediated transformation of the tetraploid emmer wheat, Triticum dicoccum. Regeneration from mature and immature embryos was accomplished as a two-step process involving callus induction in the presence of 2,4-D followed by regeneration on a 2,4-D free, cytokinin-containing medium (RM1). Higher conce...

This paper describes a protocol for the efficient vegetative propagation of Cleome rosea by somatic embryogenesis. Leaf and stem explants from nursery-grown seedlings of C. rosea were cultivated on Murashige and Skoog (MS) medium supplemented with indole-3-acetic acid (IAA), α-naphthaleneacetic acid (NAA), 4-amino-3,5,6trichloropicolinic acid (picloram) or 2,4-dichlorophenoxyacetic acid (2,4-D)...

In the present study, we established transgenic calli of Vitis amurensis that expressed a constitutively active, Ca-independent form of the AtCPK1 gene (AtCPK1-Ca) and calli with a non-active form of the gene. High-performance liquid chromatography with UV and highresolution mass-spectrometry revealed that the predominant metabolites synthesized in our transgenic callus cultures were trans-resv...

Present report is the first direct evidence of azadirachtin production in androgenic haploid cultures of Azadirachta indica, a woody medicinal tree. Anther cultures at early-late-uninucleate stage of microspores were established on MS medium with BAP (5 μM), 2,4-D (1 μM) and NAA (1 μM) containing 12% sucrose. The calli, induced, were further multiplied on 2,4-D and Kinetin media. Shoots, differ...