in order to optimize callus induction in pistacia atlantica desf. subsp. kurdica rech. f., the effect of different plant growth regulator (pgr) combinations were studied. inflorescence axis explants were cultured on wpm medium supplemented with 1, 2 and 3 mg/l of tdz or 1, 2 and 3 mg/l of ba, in combination with 1mg/l different auxins: 2, 4-d,  naa  and iba . callus initiation was observed in a...

Investigations were carried out to determine antimicrobial and antioxidant properties and total phenol content of three wild species of Ephedra compared with their respective callus cultures. Callus induction was performed in a standard Murashige and Skoog (MS) medium with the following hormonal ranges (mg/L) for every species NAA:1.5, Kin:1 for Ephedra strobiliacea, NAA:2, Kin:1 for Ephedra pr...

BACKGROUND Tea is an economic important crop with high medicinal value due to rich polyphenols content. In the present research we studied the accumulation of polyphenols of in vitro regenerated callus from anthers. OBJECTIVE Callus induction of tea anthers and in vitro accumulation of phenolic compounds from the anther-derived callus. MATERIALS AND METHODS Standardization of callus inducti...

An efficient protocol was developed for the induction of callus, somatic embryogenesis and regeneration response of two sugarcane varieties: HSF-243 and HSF-245, in this study. Callus was induced on young leaf explants cultured on Murashige and Skoog’s medium supplemented with different concentration of 2, 4-D singly or in combination with BAP. The highest percentage of callus induction was obs...

BACKGROUND Chlorophytum borivilianum is an industrially valued medicinal crop. Propagation through seeds is not feasible because of low germination percentage and long dormancy period. Therefore, callus culture and plant regeneration can be an alternative to improve this crop production. Also, callus can serve as an alternative source of bioactive compounds. OBJECTIVE To evaluate the effect o...

Barley is an important cereal crop worldwide. Its genetic transformation now limited to very few cultivars because of the high genotype dependence embryogenic callus. To reveal key genes or factors controlling callus induction and plantlet regeneration in barley, we compared transcriptomic profiles immature embryos Golden Promise ZU9, which differed dramatically efficiency transformation. The s...

The seeds of ten different genotypes of chickpea (Cicer arietinum L.) originated from Egypt, ICARDA and Saudi Arabia, were used to screen their tissue culture response on various media combination. MS media supplemented with 1, 2 and 4 mg of each of IBA and transzeatin /l, were tested. The results showed that stem results were far better from leaves explants. The callus induction ranged from 6....

The experiment was conducted during May to December 2008 in the Biotechnology Laboratory of Bangladesh Agricultural University, Mymensingh to observe the callus induction, regeneration potentiality and to establish a suitable in vitro plantlet regeneration protocol of Corchorus olitorius. MS medium supplemented with different phytohormone concentrations and combinations were used to observe the...