background and objectives: baculovirus can be used as a vector in gene delivery system. viral envelope of baculovirus would display expressed protein/peptide and it could render as a potential vaccine delivery system. in this regard, the gene coding for a subunit of shiga toxin (stxa) from escherichia coli ( e. coli ) strain was cloned in a baculovirus expression system. stxa subunit has the ab...

Nature Biotechnology’s annual survey highlights university startups that are, among other things, rethinking how to deliver gene-editing therapy and tackling various metabolic conditions, immune disorders cancer with microbiome treatments or immunotherapy. Michael Eisenstein, Ken Garber, Esther Landhuis, Caroline Seydel Laura DeFrancesco report.

The site-specific modification of an existing gene is known as editing. A section DNA must be cut with endonuclease (such the CRISPR-Cas9 system) before two severed ends are brought together, frequently a new or improved sequence inserted between them. Somatic cell editing can helpful in variety clinical contexts, and some preliminary preclinical trials have been carried out. Extremely high lev...

results this study shows the prepared gene construct is inducible by glucose. gene expression of preproinsulin was observed in muscle tissue of rabbits. conclusions these finding indicated that research in diabetes mellitus gene therapy could be performed on larger animals. objectives the purpose of this study was to construct glucose inducible insulin gene plasmid and use it in the muscle tiss...