CHROMagar Candida is a new differential culture medium that allows selective isolation of yeasts and simultaneously identifies colonies of Candida albicans, Candida glabrata, Candida tropicalis and Candida krusei. We evaluated this medium and compared it with a reference medium, Sabouraud glucose agar, for the presumptive identification of yeast species isolated directly on the medium from 1150...

BACKGROUND Both the Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) have MIC clinical breakpoints (CBPs) for fluconazole (FLU) and Candida. EUCAST CBPs are species-specific, and apply only to C. albicans, C. tropicalis and C. parapsilosis, while CLSI CBPs apply to all species. We reassessed the CLSI CBPs for FLU and ...

The study involved 100 yeast isolates, obtained from urine samples provided by a Public Pediatric Hospital of São Paulo, Brazil, from 1999 to 2004. The most frequent species was Candida albicans, followed by C. tropicalis, C. glabrata and C. parapsilosis. In regard to virulence, 97% of the isolates showed index 3 for proteinase and 63% index 2 for phospholipase. The most frequent killer biotype...

Aim: To evaluate fluconazole resistance among C. tropicalis by broth micro dilution methodand relative expression of ergosterol(ERG11) gene in resistant and sensitive Candida tropicalis. Methods: The study design was comparative conducted inMicrobiology Department UHS.A sum up 66 confirmed isolates Candidatropicalis were attained from Jinnah Hospital, Lahore. fluconazoleresistance the determine...

Objective: One of the major clinical problems is the resistance of Candida speciestowards most of antifungals. The search for new antifungal drug from plants have helped to overcome this problem. This study evaluated the effects of Allium hirtifolium and Allium sativumextracts on Candida tropicalis both in vitro and in a mouse model of systemic candidiasis. Materials and Methods: In this study,...

Demystification of microbial behaviour in mixed biofilms could have a major impact on our understanding of infectious diseases. The objectives of this study were to evaluate in vitro the interactions of six different Candida species and a Gram-negative coliform, Escherichia coli, in dual-species biofilms, and to assess the effect of E. coli LPS on Candida biofilm formation. A single isolate of ...

Biosurfactants are amphipathic molecules with both hydrophilic and hydrophobic moieties and are surface active agent that are produced extracellularly or as a part of cell membrane by bacteria, yeast and fungi. In the present study, six different microorganisms were isolated from oil contaminated soils. Screening of biosurfactant producing potential strain was done using oil spreading method, o...

Candida yeasts are the dominant fungi in the healthy human microbiome, but are well-known for causing disease following a variety of perturbations. Evaluation of fungal populations from the healthy human gut revealed a significant negative correlation between the foodborne yeast, Debaryomyces hansenii, and Candida species. D. hansenii is reported to produce killer toxins (mycocins) effective ag...

Due to the rising importance of Candida glabrata and other non-albicans Candida as principle human opportunistic pathogens, 356 women with abnormal vaginal discharge who attended a private obstetrics and gynecology clinic in Amman, Jordan, between January 1999 and February 2001 were examined. The isolation rate of Candida spp. from high-vaginal swabs was 44.9%. CHROMagar Candida and conventiona...

background: nowadays candida albicans has become resistant to the toxic and expensive commercial anti-candida drugs. therefore, investigation for new anti-fungal agents is necessary. objectives: the purpose of this survey was to investigate the in vitro anti-candida activity of the hydroalcoholic extracts of heracleum persicum fruit. materials and methods: the plant ingredients were extracted u...