1. Carbonic anhydrase was measured in tissues of silkmoths, Hyalophora cecropia, reared on either a wheatgerm-based synthetic diet or wild cherry foliage in feeding fifth-instar larvae, throughout the larval-pupal transformation and in newly ecdysed pupae. 2. Carbonic anhydrase activity was present in fat body, midgut and intgeumentary epithelial cells, but not in haemolymph, cuticle or the int...

alpha-Crystallin, a multimeric protein, exhibits chaperone-like activity in preventing aggregation of several proteins. We have studied the chaperone-like activity of alpha-crystallin toward heat-induced aggregation of bovine and human carbonic anhydrase. Human carbonic anhydrase aggregates at 60 degrees C, while bovine carbonic anhydrase does not aggregate significantly at this temperature. Re...

Two major components of carbonic anhydrase were purified from porcine red cells by column chromatography and electrofocusing techniques. Both forms behaved as single components in sedimentation velocity experiments and during starch gel electrophoresis. The observed molecular weight of both forms was about 3 x lo*. On the basis of their specific COZ hydrase activities and ammo acid compositions...

carbonic anhydrase III with an estimated purity of greater than 95%, as judged by electrophoresis in sodium dodecyl sulphate/polyacrylamide gels. Ion-exchange chromatography and salt fractionation were used to purify the bovine carbonic anhydrase 111. Bovine muscle was homogenized and adjusted t o 40% saturation with (NH4)*S04. The supernatant was applied t o a DEAE-cellulose column (2.5cm x 25...

Bundle sheath cells from leaves of a variety of C(4) species contained little or no carbonic anhydrase activity. The proportion of total leaf carbonic anhydrase in extracts of bundle sheath cells closely reflected the apparent mesophyll cell contamination of bundle sheath cell extracts as measured by the proportion of the mesophyll cell marker enzymes phosphoenolpyruvate carboxylase and pyruvat...

keeping in view the promising potential of carbonic anhydrase inhibitor based antimicrobials and enhancement of carbonic anhydrase inhibitory activity by metal complexes of sulfonamides, with an aim to develop better antimicrobial agents we have attempted investigation of antimicrobial activity of metal complexes of 4-(2'-hydroxy phenyl imino) phenyl sulphonamide. sulfanilamide was taken a...