Two genes, accB and accE, that form part of the same operon, were cloned from Streptomyces coelicolor A3(2). AccB is homologous to the carboxyl transferase domain of several propionyl coezyme A (CoA) carboxylases and acyl-CoA carboxylases (ACCases) of actinomycete origin, while AccE shows no significant homology to any known protein. Expression of accB and accE in Escherichia coli and subsequen...

Most physiology comparisons of C3 and C4 plants are made under current or elevated concentrations of atmospheric CO2 which do not reflect the low CO2 environment under which C4 photosynthesis has evolved. Accordingly, photosynthetic nitrogen (PNUE) and water (PWUE) use efficiency, and the activity of the photosynthetic carboxylases [Rubisco and phosphoenolpyruvate carboxylase (PEPC)] and decarb...

Biotin-dependent carboxylases are ubiquitous enzymes necessary for a variety of anabolic and catabolic pathways. In plants, six biotinylated polypeptides have been detected (Wurtele and Nikolau, 1990), and four biotin-dependent carboxylase enzymes have been demonstrated: acetyl-COA carboxylase (Egli et al., 1993, and refs. therein), MCCase, pyruvate carboxylase, and propionyl-COA carboxylase (W...

Biotin (vitamin B7) is a water-soluble vitamin used as co-enzyme for carboxylases essential human metabolism. The high affinity to streptavidin makes biotin an important substance in immunoassays. Excessive intake due over-the counter supplements has become problematic because of the effects on laboratory test results. There have been no reports biotin-induced thyroid immunoassay interference K...