Three complementary DNA encoding S19 ribosomal protein (S19), laminin-binding protein (LBP), and HLA class I (HLA-I) genes were isolated from a colon tumor-enriched subtraction library. To evaluate this mRNA expression, surgically removed colon tumors as well as matched normal tissue and human colon carcinoma cell lines showing various differentiation states, anchorage dependence, and prolifera...

There is now general agreement that neoplastic changes occur more frequently in the colon involved with ulcerative colitis than in the undiseased colon. We have reviewed the records of all patients with chronic ulcerative colitis and carcinoma of the colon seen at The University of Chicago hospitals and clinics to determine if there is any characteristic radiological appearance of the colon in ...

Four major carcinoembryonic antigen-related glycopeptides (Mr 180,000, 160,000, 50,000, and 40,000) were detected in SW948 colon carcinoma cells and in colon adenocarcinoma tissue using a monoclonal antibody (C(4)20-32) generated by immunizing mice with SW1222 human colon carcinoma cells. Only the Mr 50,000 polypeptide was immunoprecipitated from normal colon mucosa by this antibody. Binding st...

The aim of the study was to explore the usefulness of adenosine deaminase (ADA) activity as a marker of colon tumor progression and to find a quantitative expression of ADA in mucosa of colon carcinoma, polyps, adjacent to tumors, or mucosa from anastomosis after surgical resection. The highest ADA activity was obtained from mucosa adjacent to carcinoma compared to healthy tissue. The ADA activ...

Although gene-to-gene analyses identified genetic alterations such as APC, KRAS and TP53 mutations in colon adenomas, it is largely unknown whether there are any others in them. Mutational profiling of high-grade colon adenoma (HGCA) that just precedes colon carcinoma might identify not only novel adenoma-specific genes but also critical genes for its progression to carcinoma. For this, we perf...

We measured the in vitro protein-tyrosine kinase activity of pp60C-" from human colon carcinoma cell lines and tumors. The activity of pp6O¢-'Cl from six of nine carcinoma cell lines was higher (on average, fivefold as measured by enolase phosphorylation, or eightfold as measured by autophosphorylation) than that of pp6OC" from normal colonic mucosal cells, or human or rodent fibroblasts. Simil...

Increased expression levels of survivin are crucial for invasion activity in several types of human cancer, including colon carcinoma. However, the molecular mechanisms whereby survivin regulates cancer invasion have not been completely elucidated. To the best of our knowledge, this study is the first to investigate the role of matrix metalloprotease‑7 (MMP‑7) in cell invasion that is induced b...