This study evaluated the clastogenic and/or aneugenic potential of three nucleoside reverse transcriptase inhibitors (zidovudine - AZT, lamivudine - 3TC and stavudine - d4T) using the cytokinesis-block micronucleus (CBMN) assay in human lymphocyte cultures. All three inhibitors produced a positive response when tested in binucleated cells. The genotoxicity of AZT and 3TC was restricted to binuc...

Our previous studies demonstrated the cytogenetic effects in the peripheral blood lymphocytes of a 34-year-old male patient who received ablative radioactive 131iodine therapy (RIT) on two different occasions in 1992 and 1994. Assessment of RIT-induced chromosomal damage by the cytokinesis-blocked micronucleus assay (CBMN) showed the persistence of elevated micronucleus frequency in this patien...

INTRODUCTION Radiosensitivity is an inherent trait, associated with a raised reaction to ionizing radiation on the human body. In radiotherapy and radiation protection fields, individualization of the patient's treatment is one of the main topics. With the goal of determining biomarkers capable of anticipating normal tissue side reactions, we studied the association between the Rh factor and ra...

We performed a comprehensive study on the genotoxic and cytotoxic effects of in vitro folic acid deficiency on primary human lymphocytes. Lymphocytes were cultured in medium containing 12-120 nM folic acid for 9 days in a novel cytokinesis-block micronucleus (CBMN) assay system (n = 20). Besides identifying optimal folic acid concentrations for in vitro genomic stability, we tested the hypothes...

BACKGROUND Ionizing radiation causes detrimental health effects such as cancer and genetic damage. The study aim was to determine predictors for micronuclei (MN) occurrence and frequency in peripheral blood lymphocytes of health workers professionally exposed to radiation. METHODS Health workers, age matched, selected for the study on regular check-ups, were divided according to the radiation...

Objectives: L-carnitine has been used as therapy in hemodialysis patients for many years. When HCV was present, higher rates of patient mortality displayed. However, no study is concerned with the synergistic effect of L-carnitine and HCV. The purpose of this study was to investigate the effect of L-carnitine on the markers of DNA damage and oxidative stress in maintenance hemodialysis patients...

Thymoquinone (TQ) is the main active ingredient extracted from the black seed (Nigella sativa L.) Previous work on the antigenotoxic properties of TQ in animal cell systems has yielded inconsistent results. We investigated the antigenotoxic effect of different concentrations of TQ in human lymphocyte cultures challenged with two standard mutagens: benzo[a]pyrene (B[a]P; 16 μM), and mitomycin C ...

Chromosomal instability could be one of primary causes for malignant cell transformation. The objective of the present study was to evaluate the spontaneous genetic damages in circulated lymphocytes of newly diagnosed cancer patients by using cytokinesis-block micronucleus (CBMN) assay, with respect to the factors that might affect micronucleus frequency (i.e. age, gender, smoking habits and ca...

Ecole Normale Supérieure, Département de France. E-mail: [email protected] UPMC, 4, Place Jussieu, 75005 Paris, Franc Laboratoire des Biomolécules, CNRS, UMR7 SMIS, Synchrotron SOLEIL Saint-Aubin, 91 DISCO, Synchrotron SOLEIL Saint-Aubin, 9 Department of Physics, University of Wisco USA CNRS-Université Bordeaux 1-IPB, CBMN U 33600 Pessac, France Laboratoire Friedel, Chimie ParisTech, CN C...

Different concentrations of H. rhodopensis total extract (HRE; 0.03, 0.06 and 0.12 g/kg body weight) were injected im, into rabbits 2 h before collecting the blood samples. The whole blood samples were exposed in vitro to 2.0 Gy 60Co gamma-radiation. The radiation-induced changes were estimated by using the chromosome aberration test (CA) and cytokinesis blocked micronucleus assay (CBMN) in per...